Genome organisation and comparative genomics of four novel Wolbachia genome assemblies from Indian Drosophila host

Functional & Integrative Genomics
Kopal Singhal, Sujata Mohanty

Abstract

Wolbachia has long been known to share an endosymbiotic relationship with its host as an obligate intracellular organism. Wolbachia diversity as different supergroups is found to be host-specific in most cases except a few, where the host species is seen to accommodate multiple strains. Besides, the Wolbachia genome must have undergone several changes in response to the evolving host genome in order to adapt and establish a strong association with its host, thus making a distinctive Wolbachia-host alliance. The present study focusses on four novel genome assembly and genome-wide sequence variations of Indian Wolbachia strains, i.e. wMel and wRi isolated from two different Drosophila hosts. The genome assembly has an average size of ~ 1.1 Mb and contains ~ 1100 genes, which is comparable with the previously sequenced Wolbachia genomes. The comparative genomics analysis of these genomes and sequence-wide comparison of some functionally significant genes, i.e. ankyrin repeats, Wsp and T4SS, highlight their sequence similarities and dissimilarities, further supporting the strain-specific association of Wolbachia to its host. Interestingly, some of the sequence variations are also found to be restricted to only Indian Wolbachia stra...Continue Reading

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Methods Mentioned

BETA
PCR
protein folding

Software Mentioned

Velvet assembler
BLAST
Trimmomatic
RAST
Gene Mark tool
BASys
RAST ( Rapid Annotation using Subsystem Technology )
FIGfams
SEED VIEWER
MUSCLE

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