Genome organization and molecular characterization of the three Formica exsecta viruses-FeV1, FeV2 and FeV4

PeerJ
Kishor DhaygudeLiselotte Sundström

Abstract

We present the genome organization and molecular characterization of the three Formica exsecta viruses, along with ORF predictions, and functional annotation of genes. The Formica exsecta virus-4 (FeV4; GenBank ID: MF287670) is a newly discovered negative-sense single-stranded RNA virus representing the first identified member of order Mononegavirales in ants, whereas the Formica exsecta virus-1 (FeV1; GenBank ID: KF500001), and the Formica exsecta virus-2 (FeV2; GenBank ID: KF500002) are positive single-stranded RNA viruses initially identified (but not characterized) in our earlier study. The new virus FeV4 was found by re-analyzing data from a study published earlier. The Formica exsecta virus-4 genome is 9,866 bp in size, with an overall G + C content of 44.92%, and containing five predicted open reading frames (ORFs). Our bioinformatics analysis indicates that gaps are absent and the ORFs are complete, which based on our comparative genomics analysis suggests that the genomes are complete. Following the characterization, we validate virus infection for FeV1, FeV2 and FeV4 for the first time in field-collected worker ants. Some colonies were infected by multiple viruses, and the viruses were observed to infect all castes, a...Continue Reading

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Citations

Jan 8, 2021·Virology Journal·César Augusto Diniz XavierAnna Elizabeth Whitfield
Nov 6, 2021·The Journal of General Virology·Ralf G Dietzgen Ictv Report Consortium
Dec 5, 2020·Virus Research·Daisuke KobayashiHaruhiko Isawa

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Datasets Mentioned

BETA
KF500002
MF287670
MF287660
MF287663
MF287664
MF287668
MF287669
Length

Methods Mentioned

BETA
RNAseq
PCR
electrophoresis
RNA-Seq

Software Mentioned

Samtools
blast
bowtie aligner
Blast2Go
Velvet
hmm
RAxML
Trinity
GeneFisher
ncbi

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