Genome plasticity favours double chromosomal Tn4401b-blaKPC-2 transposon insertion in the Pseudomonas aeruginosa ST235 clone

BMC Microbiology
Deisy AbrilJavier Escobar-Perez

Abstract

Pseudomonas aeruginosa Sequence Type 235 is a clone that possesses an extraordinary ability to acquire mobile genetic elements and has been associated with the spread of resistance genes, including genes that encode for carbapenemases. Here, we aim to characterize the genetic platforms involved in resistance dissemination in blaKPC-2-positive P. aeruginosa ST235 in Colombia. In a prospective surveillance study of infections in adult patients attended in five ICUs in five distant cities in Colombia, 58 isolates of P. aeruginosa were recovered, of which, 27 (46.6%) were resistant to carbapenems. The molecular analysis showed that 6 (22.2%) and 4 (14.8%) isolates harboured the blaVIM and blaKPC-2 genes, respectively. The four blaKPC-2-positive isolates showed a similar PFGE pulsotype and belonged to ST235. Complete genome sequencing of a representative ST235 isolate shows a unique chromosomal contig of 7097.241 bp with eight different resistance genes identified and five transposons: a Tn6162-like with ant(2″)-Ia, two Tn402-like with ant(3″)-Ia and blaOXA-2 and two Tn4401b with blaKPC-2. All transposons were inserted into the genomic islands. Interestingly, the two Tn4401b copies harbouring blaKPC-2 were adjacently inserted into a...Continue Reading

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Citations

Aug 20, 2020·Expert Review of Anti-infective Therapy·Juan Carlos García-BetancurMaría Virginia Villegas
Jul 25, 2019·Antibiotics·Tatiana PachecoJavier Escobar-Perez
Oct 13, 2020·International Journal of Antimicrobial Agents·Ester Del Barrio-TofiñoAntonio Oliver
Mar 9, 2021·Frontiers in Microbiology·Eun-Jeong Yoon, Seok Hoon Jeong
May 4, 2021·Frontiers in Cellular and Infection Microbiology·R Frèdi LangendonkJoanne L Fothergill

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Methods Mentioned

BETA
PCR

Software Mentioned

RAxML
Gepard ( Genome Pair Rapid Dotter )
BLASTn
GelCompar II
Tablet
ARIBA
ACT
ANNOT ANNOTation
ISfinder
Prokka

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