Genome Plasticity in Cultured Leishmania donovani : Comparison of Early and Late Passages

Frontiers in Microbiology
Roma SinhaNahid Ali

Abstract

Leishmania donovani possesses a complex heteroxenic life cycle where infective metacyclic promastigotes are pre-adapted to infect their host and cope up with intracellular stress. Exploiting the similarities between cultured and sandfly derived promastigotes, we used early and late passage cultured promastigotes to show specific changes at genome level which compromise pathogen fitness reflected in gene expression and infection studies. The pathogen loses virulence mostly via transcriptional and translational regulations and long-time cultivation makes them struggle to convert to virulent metacyclics. At the genomic level very subtle plasticity was observed between the early and the late passages mostly in defense-related, nutrient acquisition and signal transduction genes. Chromosome Copy number variation is seen in the early and late passages involving several genes that may be playing a role in pathogenicity. Our study highlights the importance of ABC transporters and calpain like cysteine proteases in parasite virulence in cultured promastigotes. Interestingly, these proteins are emerging as important patho-adaptive factors in clinical isolates of Leishmania. We found that the currently available genome of Leishmania in the...Continue Reading

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Citations

Apr 21, 2020·PLoS Neglected Tropical Diseases·James A CottonMatthew Yeo
May 31, 2020·Breast Cancer : the Journal of the Japanese Breast Cancer Society·Ayse CanerAyfer Haydaroğlu
Mar 27, 2020·ELife·Susanne U FranssenJames A Cotton
Feb 26, 2019·Frontiers in Cellular and Infection Microbiology·Mohammad ShadabNahid Ali
Nov 13, 2020·Frontiers in Cellular and Infection Microbiology·Luz H PatinoJuan David Ramírez
May 24, 2021·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Sofia El KacemMeryem Lemrani
Dec 30, 2021·PLoS Neglected Tropical Diseases·Ester PolákováVyacheslav Yurchenko

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Datasets Mentioned

BETA
PRJNA171503

Methods Mentioned

BETA
FCS
electrophoresis
RNAseq
Tryptic

Software Mentioned

MUMmer
Allpaths
Genome Analysis toolkit ( GATK )
PD Quest
PROmer
RATT
Scipio pipeline
Samtools
KASS
R scripts

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