Genome-resolved metagenomics of sugarcane vinasse bacteria

Biotechnology for Biofuels
Noriko A CassmanEiko E Kuramae

Abstract

The production of 1 L of ethanol from sugarcane generates up to 12 L of vinasse, which is a liquid waste containing an as-yet uncharacterized microbial assemblage. Most vinasse is destined for use as a fertilizer on the sugarcane fields because of the high organic and K content; however, increased N2O emissions have been observed when vinasse is co-applied with inorganic N fertilizers. Here we aimed to characterize the microbial assemblage of vinasse to determine the gene potential of vinasse microbes for contributing to negative environmental effects during fertirrigation and/or to the obstruction of bioethanol fermentation. We measured chemical characteristics and extracted total DNA from six vinasse batches taken over 1.5 years from a bioethanol and sugar mill in Sao Paulo State. The vinasse microbial assemblage was characterized by low alpha diversity with 5-15 species across the six vinasses. The core genus wasLactobacillus. The top six represented bacterial genera across the samples wereLactobacillus,MegasphaeraandMitsuokella(Phylum Firmicutes, 35-97% of sample reads);ArcobacterandAlcaligenes(Phylum Proteobacteria, 0-40%);Dysgonomonas(Phylum Bacteroidetes, 0-53%); andBifidobacterium(Phylum Actinobacteria, 0-18%). Potentia...Continue Reading

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Methods Mentioned

BETA
PCR
electrophoresis
phage therapy

Software Mentioned

Metafast
metaSPADES
GraPhlan
bowtie2
PEAR
Ray
MGRAST
RAST
hclust2
metaphlan2

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