Genome-scale approach to study the genetic relatedness among Brucella melitensis strains

PloS One
Ana PeleritoJoão Paulo Gomes

Abstract

Brucellosis is an important zoonotic disease that affects both humans and animals. To date, laboratory surveillance is still essentially based on the traditional MLVA-16 methodology and the associated epidemiological information is frequently scarce. Our goal was to contribute to the improvement of Brucella spp. surveillance through the implementation of a whole genome sequencing (WGS) approach. We created a curated ready-to-use species-specific wgMLST scheme enrolling a panel of 2656 targets (http://doi.org/10.5281/zenodo.3575026) and used this schema to perform a retrospective analysis of the genetic relatedness among B. melitensis strains causing human infection in Portugal (a country where brucellosis is an endemic disease) from 2010 to 2018. The strains showed a phylogenetic clustering within genotype II (25 out of 36) and IV (4 out of 36), and shared clades with strains isolated from countries with which Portugal has intense food trading, tourism and similar eating habits, such as Spain, Italy and Greece. In addition, our results point to the identification of strong associations between B. melitensis strains, likely underlying missed "outbreaks" as 22 out of the 36 strains showed genetic linkage with others. In fact, the...Continue Reading

References

Apr 1, 1997·Emerging Infectious Diseases·M J Corbel
Jan 5, 2002·Proceedings of the National Academy of Sciences of the United States of America·Vito G DelVecchioRoss Overbeek
Jul 20, 2004·Proceedings of the National Academy of Sciences of the United States of America·Koichiro TamuraSudhir Kumar
Jan 28, 2006·The Lancet Infectious Diseases·Georgios PappasEpameinondas V Tsianos
Mar 1, 2006·BMC Microbiology·Vladyslava G RatushnaCynthia J Gibas
Jun 8, 2006·Journal of Clinical Microbiology·Adrian M WhatmoreAlastair P MacMillan
Jan 31, 2007·Journal of Microbiological Methods·Sascha Al DahoukHeinrich Neubauer
May 20, 2009·BMC Bioinformatics·Alexandre P FranciscoJoão A Carriço
Aug 8, 2009·International Journal of Systematic and Evolutionary Microbiology·Holger C ScholzBarun Kumar De
Apr 18, 2012·Journal of Computational Biology : a Journal of Computational Molecular Cell Biology·Anton BankevichPavel A Pevzner
Aug 21, 2012·PloS One·Ana Cristina FerreiraMaria Inácia Corrêa de Sá
Dec 18, 2013·Journal of Bacteriology·Alice R WattamDavid O'Callaghan
Apr 4, 2014·Bioinformatics·Anthony M BolgerBjoern Usadel
May 27, 2014·Frontiers in Microbiology·Edgardo Moreno
Sep 23, 2014·International Journal of Systematic and Evolutionary Microbiology·Adrian M WhatmoreNatalia E Schlabritz-Loutsevitch
Feb 10, 2016·Frontiers in Microbiology·Jens A HammerlStefan Hertwig
Mar 2, 2016·International Journal of Systematic and Evolutionary Microbiology·Holger C ScholzErwin Hofer
Mar 24, 2016·Molecular Biology and Evolution·Sudhir KumarKoichiro Tamura
May 1, 2016·Nucleic Acids Research·Bruno Ribeiro-GonçalvesJoão André Carriço
Dec 3, 2016·Antimicrobial Resistance and Infection Control·Hsi LiuDavid Trees
Jul 1, 1985·Evolution; International Journal of Organic Evolution·Joseph Felsenstein
Sep 30, 2017·FEMS Microbiology Letters·Natalia Abou ZakiSima Tokajian
Nov 21, 2017·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Jagadesan SankarasubramanianJeyaprakash Rajendhran
May 12, 2018·BMC Genomics·Sergey V PisarenkoAlexander N Kulichenko
Nov 6, 2018·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Jagadesan SankarasubramanianJeyaprakash Rajendhran

❮ Previous
Next ❯

Citations

Mar 16, 2021·Frontiers in Medicine·Justine SchaefferWerner Ruppitsch
Apr 20, 2021·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Adrian M Whatmore, Jeffrey T Foster
Jun 21, 2021·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Inês SilvestreMaria José Borrego

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR

Software Mentioned

BLAST
SPAdes
MEGA7
PHYLOViZ desktop
PHYLOViZ
snippy
Snippy4
goeBURST
Trimmomatic
Pilon

Related Concepts

Related Feeds

Brucellosis

Brucellosis is a bacterial infection caused by members of the genus brucella and remains one of the world's major zoonotic diseases. Discover the latest research on Brucellosis here.

Brucellosis (ASM)

Brucellosis is a bacterial infection caused by members of the genus brucella and remains one of the world's major zoonotic diseases. Discover the latest research on Brucellosis here.

Related Papers

Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases
Alexandr ShevtsovKasim Mukanov
Nederlands tijdschrift voor geneeskunde
J G Borghans, B L Buennemeyer
American Journal of Public Health and the Nation's Health
I F Huddleson, M Munger
© 2021 Meta ULC. All rights reserved