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Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening

Nature Protocols

Apr 1, 2017

Julia JoungFeng Zhang

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Abstract

Forward genetic screens are powerful tools for the unbiased discovery and functional characterization of specific genetic elements associated with a phenotype of interest. Recently, the RNA-guided endonuclease Cas9 from the microbial CRISPR (clustered regularly interspaced short palindr...read more

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  • Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening

    Nature Protocols

    Apr 1, 2017

    Julia JoungFeng Zhang

    PMID: 28333914

    DOI: 10.1038/nprot.2017.016

    Abstract

    Forward genetic screens are powerful tools for the unbiased discovery and functional characterization of specific genetic elements associated with a phenotype of interest. Recently, the RNA-guided endonuclease Cas9 from the microbial CRISPR (clustered regularly interspaced short palindr...read more

    39
    1176
    1

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