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Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening

Nature Protocols

Mar 24, 2017

Julia JoungFeng Zhang

Abstract

Forward genetic screens are powerful tools for the unbiased discovery and functional characterization of specific genetic elements associated with a phenotype of interest. Recently, the RNA-guided endonuclease Cas9 from the microbial CRISPR (clustered regularly interspaced short palindr...read more

Mentioned in this Paper

Immune System
Subfamily lentivirinae
Shuttle Vectors
CRISPR-Cas Systems
Endonuclease
Candidate Disease Gene
Genetic Screening (Procedure)
Genomic Screening
Genomics
Microbial
39
1179
1
1
Paper Details
References
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  • Citations42
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  • References74
  • Citations42
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Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening

Nature Protocols

Mar 24, 2017

Julia JoungFeng Zhang

PMID: 28333914

DOI: 10.1038/nprot.2017.016

Abstract

Forward genetic screens are powerful tools for the unbiased discovery and functional characterization of specific genetic elements associated with a phenotype of interest. Recently, the RNA-guided endonuclease Cas9 from the microbial CRISPR (clustered regularly interspaced short palindr...read more

Mentioned in this Paper

Immune System
Subfamily lentivirinae
Shuttle Vectors
CRISPR-Cas Systems
Endonuclease
Candidate Disease Gene
Genetic Screening (Procedure)
Genomic Screening
Genomics
Microbial
39
1179
1
1

Similar Papers Found In These Feeds

CRISPR for Genome Editing

Genome editing technologies enable the editing of genes to create or correct mutations. Clustered regularly interspaced short palindromic repeats (CRISPR) are DNA sequences in the genome that are recognized and cleaved by CRISPR-associated proteins (Cas). Here is the latest research on the use of CRISPR-Cas system in gene editing.

Immune System in Humans: Tissues & Ages

Mapping the human immune system spatially, within and between tissues, will provide the basis for studying mechanisms of tissue residency and the effects of aging. Find new research on analysis of the immune system by multiple approaches here.

Related Papers

Nature Reviews. Genetics

High-throughput functional genomics using CRISPR-Cas9

Nature Reviews. GeneticsApril 10, 2015
Ophir ShalemFeng Zhang
Nature Protocols

Genome engineering using the CRISPR-Cas9 system

Nature ProtocolsOctober 26, 2013
F Ann RanFeng Zhang
Paper Details
References
  • References74
  • Citations42
12345...
  • References74
  • Citations42
12345
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