Genome sequence analysis of a Helicoverpa armigera single nucleopolyhedrovirus (HearNPV-TR) isolated from Heliothis peltigera in Turkey

PloS One
Gözde Büşra EroğluZihni Demirbağ

Abstract

The entire genome of Helicoverpa armigera single nucleopolyhedrovirus (HearNPV-TR) was sequenced, and compared to genomes of other existing isolates. HearNPV-TR genome is 130.691 base pairs with a 38.9% G+C content and has 137 open reading frames (ORFs) of ≥ 150 nucleotides. Five homologous repeated sequences (hrs) and two baculovirus repeated ORFs (bro-a and bro-b) were identified. Phylogenetic analysis showed that HearNPV-TR is closer to HaSNPV-C1, HaSNPV-G4, HaSNPV-AU and HasNPV. However, there are significant differences in hr3, hr5 regions and in bro-a gene. Pairwise Kimura-2 parameter analysis of 38 core genes sequences of HearNPV-TR and other Helicoverpa NPVs showed that the genetic distances for these sequences were below 0.015 substitutions/site. Genomic differences as revealed by restriction profiles indicated that hr3, hr5 regions and bro-a gene may play a role in the virulence of HearNPV-TR.

References

Sep 1, 1988·Virology·I R Smith, N E Crook
Sep 1, 1995·The Journal of General Virology·M KoolG F Rohrmann
Sep 30, 1999·Virology·T HayakawaS Maeda
Nov 24, 1999·The Journal of General Virology·W F IJkelJ M Vlak
Apr 21, 2001·Cell Death and Differentiation·R J Clem
Aug 3, 2001·Journal of Virology·E A HerniouD R O'Reilly
Sep 20, 2001·The Journal of General Virology·T LuqueD Winstanley
Nov 5, 2002·Annual Review of Entomology·Elisabeth A HerniouDavid R O'Reilly
Jun 18, 2003·The Journal of General Virology·Robert L Harrison, Bryony C Bonning
Jul 5, 2003·Virology·Sally WormleatonDoreen Winstanley
Nov 15, 2003·Journal of Invertebrate Pathology·C ReedH L Williams
Dec 31, 2003·Virology·Martin Lange, Johannes A Jehle
Jun 15, 2004·Journal of Virology·Hilary A M LauzonBasil M Arif
Jun 17, 2005·The Journal of General Virology·Monique M van OersJust M Vlak
Nov 30, 2005·Virology·Johannes A JehleRüdiger Hauschild
Jun 9, 2006·The Journal of General Virology·Shannon R EscasaBasil M Arif
Apr 7, 2007·The Journal of General Virology·Sally Hilton, Doreen Winstanley
Nov 23, 2013·Biotechnology Progress·A Contreras-GómezY Chisti
Nov 4, 2016·Viruses·Christopher Noune, Caroline Hauxwell

❮ Previous
Next ❯

Datasets Mentioned

BETA
KJ909666
MK507817
AXN77341

Methods Mentioned

BETA
electrophoresis

Software Mentioned

BioEdit
Benchling
Unicycler
Benchling Biology
Pilon
Benchling Biology Software
MEGA6

Related Concepts

Related Feeds

Cell Adhesion Molecules in the Brain

Cell adhesion molecules found on cell surface help cells bind with other cells or the extracellular matrix to maintain structure and function. Here is the latest research on their role in the brain.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.