Genome sequence and comparative microarray analysis of serotype M18 group A Streptococcus strains associated with acute rheumatic fever outbreaks

Proceedings of the National Academy of Sciences of the United States of America
J C SmootJames M Musser

Abstract

Acute rheumatic fever (ARF), a sequelae of group A Streptococcus (GAS) infection, is the most common cause of preventable childhood heart disease worldwide. The molecular basis of ARF and the subsequent rheumatic heart disease are poorly understood. Serotype M18 GAS strains have been associated for decades with ARF outbreaks in the U.S. As a first step toward gaining new insight into ARF pathogenesis, we sequenced the genome of strain MGAS8232, a serotype M18 organism isolated from a patient with ARF. The genome is a circular chromosome of 1,895,017 bp, and it shares 1.7 Mb of closely related genetic material with strain SF370 (a sequenced serotype M1 strain). Strain MGAS8232 has 178 ORFs absent in SF370. Phages, phage-like elements, and insertion sequences are the major sources of variation between the genomes. The genomes of strain MGAS8232 and SF370 encode many of the same proven or putative virulence factors. Importantly, strain MGAS8232 has genes encoding many additional secreted proteins involved in human-GAS interactions, including streptococcal pyrogenic exotoxin A (scarlet fever toxin) and two uncharacterized pyrogenic exotoxin homologues, all phage-associated. DNA microarray analysis of 36 serotype M18 strains from di...Continue Reading

References

Apr 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·J M MusserR K Selander
Oct 20, 1995·Science·C M FraserJ C Venter
Apr 1, 1996·The Journal of Infectious Diseases·M S ChausseeJ J Ferretti
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Mar 30, 1999·Molecular Microbiology·A PodbielskiK H Schmidt
Apr 30, 1999·Emerging Infectious Diseases·R FacklamG Tyrrell
Apr 30, 1999·Emerging Infectious Diseases·N HoeJ M Musser
May 15, 1999·Nucleic Acids Research·A L DelcherS L Salzberg
Nov 11, 1999·Nucleic Acids Research·A L DelcherS L Salzberg
Feb 24, 2000·Nucleic Acids Research·T D ReadC M Fraser
Jul 25, 2000·Clinical Microbiology Reviews·M W Cunningham
Dec 20, 2000·Proceedings of the National Academy of Sciences of the United States of America·N SalamaS Falkow
Apr 3, 2001·Genome Research·D GordonP Green
Apr 11, 2001·Proceedings of the National Academy of Sciences of the United States of America·J J FerrettiR McLaughlin
Jul 12, 2001·Proceedings of the National Academy of Sciences of the United States of America·J R FitzgeraldJ M Musser
Aug 23, 2001·Proceedings of the National Academy of Sciences of the United States of America·L M SmootJ M Musser
Sep 7, 2001·Journal of Bacteriology·J HoskinsJ I Glass

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Citations

Jul 17, 2008·Comparative and Functional Genomics·Jamila ShafiMichael R Barer
Mar 29, 2005·Journal of Molecular Evolution·Francisco J M MojicaElena Soria
Oct 30, 2009·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·E A AhmedJ B Dale
Feb 3, 2006·Functional & Integrative Genomics·Jodi A Lindsay, Matthew T G Holden
Apr 15, 2004·Current Opinion in Biotechnology·Stephen McGrathDouwe van Sinderen
Nov 30, 2005·The American Journal of Pathology·James M Musser, Frank R DeLeo
Mar 7, 2003·FEMS Microbiology Letters·Awdhesh Kalia, Debra E Bessen
Nov 16, 2002·Current Opinion in Genetics & Development·Thomas S Whittam, Alyssa C Bumbaugh
Jun 29, 2002·Trends in Microbiology·Mohammed SebaihiaJulian Parkhill
Sep 10, 2002·Trends in Microbiology·Nicholas ThomsonJulian Parkhill
Apr 23, 2003·Trends in Microbiology·Jean-Pierre Claverys, Bernard Martin
Nov 5, 2002·Current Opinion in Chemical Biology·David R Walt
Aug 28, 2003·Current Opinion in Microbiology·Carlos CanchayaHarald Brüssow
Apr 8, 2003·The Lancet Infectious Diseases·A L BisnoC M Collins
Feb 12, 2004·The Lancet Infectious Diseases·Penelope A BryantNigel Curtis
Nov 1, 2005·The Journal of Molecular Diagnostics : JMD·Kenneth L MuldrewYi-Wei Tang
Mar 24, 2004·Nature Reviews. Microbiology·Timothy J Mitchell
Sep 28, 2007·Natural Product Reports·Stefano DonadioMargherita Sosio
Oct 2, 2002·Molecular Microbiology·Philippe GlaserFrank Kunst
Oct 9, 2002·Molecular Microbiology·Carlos Hidalgo-GrassEmanuel Hanski
Jan 20, 2006·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Stanford T ShulmanRobert R Tanz
Jan 20, 2006·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Grace M Lee, Michael R Wessels
Jan 15, 2008·The Journal of Infectious Diseases·Torsten G LoofEva Medina
Jan 9, 2008·The Journal of Infectious Diseases·Ivo VojtekEmmanuelle Charpentier
May 1, 2008·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Gene H Stollerman, James B Dale
Jul 27, 2007·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Thomas ProftJohn D Fraser
Oct 5, 2002·Nucleic Acids Research·Jan MrázekSamuel Karlin
Jun 4, 2005·Nucleic Acids Research·Emmanuelle Lerat, Howard Ochman
Jan 27, 2004·The Pediatric Infectious Disease Journal·Lonnie J MinerUNKNOWN Post-Streptococcal Syndrome Stydy Team
Feb 22, 2003·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·W F DoolittleA J Roger
Nov 19, 2008·Genome Research·Xavier DidelotDaniel Falush
Apr 7, 2006·Applied and Environmental Microbiology·Cordula LembkeBernd Kreikemeyer
Jan 18, 2005·Clinical Microbiology Reviews·Gunnar LindahlThomas Areschoug

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