Abstract
Background and objectives: Extended-spectrum β-lactamase (ESBL)-producing Klebsiella pneumoniae is a serious public health issue globally. In this study, the antibiotic resistance genes, virulence factors, mobile genetic elements, and genetic lineages of circulating ESBL-producing K. pneumoniae strains isolated from pigs and humans in Cameroonian abattoirs were investigated using whole genome sequencing (WGS), in order to ascertain zoonotic transmission (viz. from animals to humans and/or vice-versa) in the food chain. Methods: During March-October 2016, 288 nasal and rectal pooled samples from 432 pigs as well as nasal and hand swabs from 82 humans were collected from Cameroon and South Africa. Seven ESBL-producing K. pneumoniae circulating in Cameroonian pig abattoirs were selected and their genomic DNA sequenced using an Illumina MiSeq platform. Generated reads were de novo assembled using the Qiagen CLC Genomics Workbench and SPAdes. The assembled contigs were annotated using RAST and antibiotic resistance genes, virulence factors, plasmids, and bacteriophages were identified with ResFinder, Virulence Finder, PlasmidFinder, and PHAST, respectively. Results: ESBL-producing K. pneumoniae were detected in pigs (34/158; 21.52%)...Continue Reading
References
Dec 25, 1991·Nucleic Acids Research·J VersalovicJ R Lupski
Dec 24, 2004·Antimicrobial Agents and Chemotherapy·J Gangoue-PiebojiL S Tzouvelekis
Aug 6, 2005·Journal of Clinical Microbiology·Laure DiancourtSylvain Brisse
Feb 12, 2008·BMC Genomics·Ramy K AzizOlga Zagnitko
Apr 21, 2010·Antimicrobial Agents and Chemotherapy·Jun-ichi WachinoYoshichika Arakawa
Mar 12, 2011·Letters in Applied Microbiology·H XuJ Davies
Jun 16, 2011·Nucleic Acids Research·You ZhouDavid S Wishart
Mar 7, 2012·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·S BreurecS Brisse
Apr 18, 2012·Journal of Computational Biology : a Journal of Computational Molecular Cell Biology·Anton BankevichPavel A Pevzner
May 25, 2012·BMC Genomics·Jason R GrantPaul Stothard
Jul 12, 2012·The Journal of Antimicrobial Chemotherapy·Ea ZankariMette Voldby Larsen
Nov 30, 2012·The Journal of Antimicrobial Chemotherapy·Jianxia HouJian-Hua Liu
May 8, 2013·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·C Lonchel MagouéP De Mol
Dec 3, 2013·Nucleic Acids Research·Ross OverbeekRick Stevens
Jan 31, 2014·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Zineb BelbelJean-Marc Rolain
Feb 28, 2014·Journal of Clinical Microbiology·Katrine Grimstrup JoensenFrank M Aarestrup
Apr 30, 2014·Antimicrobial Agents and Chemotherapy·Alessandra CarattoliHenrik Hasman
Jun 7, 2014·PloS One·Corey M HudsonKelly P Williams
Jul 2, 2014·Antimicrobial Agents and Chemotherapy·Jane ChanPak-Leung Ho
Sep 13, 2014·South African Medical Journal = Suid-Afrikaanse Tydskrif Vir Geneeskunde·Olga PerovicElse Marais
Jul 23, 2015·Bioinformatics·Andrew J PageJulian Parkhill
Sep 4, 2015·The Pan African Medical Journal·Emilia Enjema LyongaSinata Koulla-Shiro
Jan 23, 2016·South African Medical Journal = Suid-Afrikaanse Tydskrif Vir Geneeskunde·Rachael Kiera JacobsonColleen Bamford
Feb 13, 2016·Memórias do Instituto Oswaldo Cruz·Zumaana RafiqRama Vaidyanathan
Sep 27, 2016·International Journal of Microbiology·Rolf Nyah-Tuku NzalieSinata Koulla-Shiro
Oct 12, 2016·South African Medical Journal = Suid-Afrikaanse Tydskrif Vir Geneeskunde·O PerovicA Singh-Moodley
Dec 10, 2016·Frontiers in Microbiology·Luria Leslie FounouSabiha Yusuf Essack
Feb 12, 2017·Diagnostic Microbiology and Infectious Disease·Jung Ok KimKyungwon Lee
Aug 9, 2017·Microbial Genomics·Laura VillaAlessandra Carattoli
Aug 19, 2017·Emerging Infectious Diseases·Yahia BenzeraraGuillaume Arlet
Aug 31, 2017·MBio·Ryota ItoYohei Doi
Citations
May 24, 2018·Genes·Elena A OniciucAvelino Alvarez-Ordóñez
Sep 12, 2018·PloS One·Mauro M S SaraivaCelso J B Oliveira
Mar 8, 2019·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Golnaz MobasseriKwai Lin Thong
Mar 11, 2020·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Nadezhda K FursovaNikolay V Volozhantsev
Apr 17, 2020·MSphere·Dulmini Nanayakkara SapugahawatteMargaret Ip
Jan 29, 2020·Scientific Reports·Nontombi Marylucy MbelleSabiha Yusuf Essack
Dec 25, 2019·Scientific Reports·Rubén MonárrezIruka N Okeke
Mar 25, 2019·The Journal of Antimicrobial Chemotherapy·Fuguang ChenSiguo Liu
Jul 3, 2019·Zoonoses and Public Health·Clara AtterbyJosef D Järhult
Feb 12, 2021·Microorganisms·Novaria Sari Dewi PanjaitanPo-Chi Soo
Feb 5, 2021·Antibiotics·Kabiru O AkinyemiGamal Wareth
Oct 20, 2020·International Journal of Antimicrobial Agents·Oumar Ouchar MahamatSylvain Godreuil
May 1, 2021·Antibiotics·Zoi AthanasakopoulouCharalambos Billinis
Aug 14, 2021·Frontiers in Microbiology·Wandee SirichokchatchawanNuvee Prapasarakul
Nov 19, 2019·ACS Infectious Diseases·Charles E Okolie, Unyime C Essien