Genome shuffling and ribosome engineering of Streptomyces virginiae for improved virginiamycin production

Bioprocess and Biosystems Engineering
Qian-Qian TongJian-Ming Yao

Abstract

The production of virginiamycin (VGM) from Streptomyces virginiae was improved by genome shuffling and ribosome engineering companied with a high-throughput screening method integrating deep-well cultivation and the cylinder-plate detecting. First, a novel high-throughput method was developed to rapidly screen large numbers of VGM-producing mutants. Then, the starting population of genome shuffling was obtained through ultraviolet (UV) and microwave mutagenesis, and four mutants with higher productivity of VGM were selected for genome shuffling. Next, the parent protoplasts were inactivated by UV and heat when a fusant probability was about 98%. Streptomycin resistance was used as an evolutionary pressure to extend positive effects on VGM synthesis. Finally, after five rounds of genome shuffling, a genetically stable strain G5-103 was obtained and characterized to be able to yield 251 mg/L VGM, which was 3.1- and 11.6-fold higher than that of the mutant strain UV 1150 and the wild-type strain, respectively.

References

Mar 26, 1999·Applied Microbiology and Biotechnology·S ShioyaH Shimizu
Oct 13, 2000·Applied Microbiology and Biotechnology·S ParekhR J Strobel
Feb 8, 2002·Nature·Ying-Xin ZhangStephen del Cardayré
Jun 29, 2002·Nature Biotechnology·Ranjan PatnaikStephen del Cardayré
Nov 30, 2004·Advances in Applied Microbiology·Kozo OchiKazuhiko Kurosawa
Feb 11, 2005·Chemical Reviews·Tariq A Mukhtar, Gerard D Wright
Oct 18, 2006·Applied Microbiology and Biotechnology·Hiroyuki HidaY Yamada
Jun 26, 2007·Bioscience, Biotechnology, and Biochemistry·Kozo Ochi
May 19, 2009·Applied and Environmental Microbiology·Yukinori TanakaKozo Ochi
May 26, 2009·Biotechnology Advances·Jixian GongXueming Zhao
Jan 22, 2011·Journal of Industrial Microbiology & Biotechnology·Richard H Baltz
May 23, 2012·Journal of Agricultural and Food Chemistry·Jian-Mei LuoMin Wang
Aug 23, 2012·Applied Microbiology and Biotechnology·Xing-An LvXinle Liang
Nov 13, 2012·Applied Microbiology and Biotechnology·Kozo Ochi, Takeshi Hosaka
May 10, 2013·Journal of Industrial Microbiology & Biotechnology·Ji ZhangWensheng Xiang
May 17, 2013·Journal of Industrial Microbiology & Biotechnology·Pu ZhengZhihao Sun
Sep 18, 2013·Applied Biochemistry and Biotechnology·Wei LiZhiqun Liang
May 16, 2014·Applied Biochemistry and Biotechnology·Qingling WangXinle Liang
Aug 8, 2014·Applied Biochemistry and Biotechnology·Yuping ZhaoJingzhu Yang
May 15, 2015·Bioprocess and Biosystems Engineering·Yong-Peng ZhouLei Tang
Jul 17, 2016·Applied Biochemistry and Biotechnology·Liang WangZhong-Gui Mao

Related Concepts

Related Feeds

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.