Genome shuffling enhanced ε-poly-L-lysine production by improving glucose tolerance of Streptomyces graminearus

Applied Biochemistry and Biotechnology
Shu LiZhong-Gui Mao

Abstract

The productivity of ε-poly-L: -lysine (ε-PL) in currently reported wild-type strains is low. Here we improved glucose tolerance of a Streptomyces graminearus strain LS-B1 by genome shuffling while simultaneously enhancing the ε-PL productivity. The starting population was generated by ultraviolet irradiation and nitrosoguanidine mutagenesis and then subjected for recursive protoplast fusion. The positive colonies from library, created by fusing the inactivated protoplasts were screened on agar plates containing different concentrations of glucose. Characterization of all recombinants and wild-type strain in shake-flask fermentation indicated the compatibility of two phenotypes of glucose tolerance and ε-PL yield enhancement. The best performing recombinant, F3-4, was isolated after three rounds of genome shuffling, whose ε-PL production was about 88% higher than that of the parent strain. In batch fermentation test, the ε-PL concentration was obtained as 2.4 g/L by F3-4 compared with 1.6 g/L of wild type. Fed-batch fermentation by F3-4 was carried out and the ε-PL production accumulated to 13.5 g/L when initial glucose concentration was improved from 50 to 85 g/L. Enzyme activities of hexokinase, pyruvate kinase, and citrate sy...Continue Reading

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Aug 17, 2010·Journal of Industrial Microbiology & Biotechnology·Shu LiZhonggui Mao

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Citations

Oct 1, 2013·BioMed Research International·Amit Kumar ChaudharyJae Kyung Sohng
Mar 7, 2014·Applied Microbiology and Biotechnology·Damien Biot-Pelletier, Vincent J J Martin
May 15, 2015·Bioprocess and Biosystems Engineering·Yong-Peng ZhouLei Tang
Oct 26, 2014·Applied Biochemistry and Biotechnology·Qi-Xing SunZhong-Gui Mao
Jul 17, 2016·Applied Biochemistry and Biotechnology·Liang WangZhonggui Mao
Nov 4, 2016·Bioprocess and Biosystems Engineering·Liang WangZhonggui Mao
Jan 12, 2017·Applied Microbiology and Biotechnology·Chunkai GuJianan Zhang
Oct 9, 2019·Journal of Industrial Microbiology & Biotechnology·Long PanZhonggui Mao
May 24, 2013·Applied Biochemistry and Biotechnology·Chao WangXinle Liang
May 26, 2017·Applied Biochemistry and Biotechnology·Yong-Juan LiuZhong-Gui Mao
Oct 31, 2017·Bioprocess and Biosystems Engineering·Peng YanLei Tang
Oct 24, 2019·World Journal of Microbiology & Biotechnology·Ping YuTingting Yan
Apr 4, 2021·Polymers·Jonathan David HinchliffeIpsita Roy

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