Genome shuffling improves production of the low-temperature alkalophilic lipase by Acinetobacter johnsonii

Biotechnology Letters
HaiKuan WangYuJie Dai

Abstract

The production of a low-temperature alkalophilic lipase from Acinetobacter johnsonii was improved using genome shuffling. The starting populations, obtained by UV irradiation and diethyl sulfate mutagenesis, were subjected to recursive protoplast fusion. The optimal conditions for protoplast formation and regeneration were 0.15 mg lysozyme/ml for 45 min at 37°C. The protoplasts were inactivated under UV for 20 min or heated at 60°C for 60 min and a fusant probability of ~98% was observed. The positive colonies were created by fusing the inactivated protoplasts. After two rounds of genome shuffling, one strain, F22, with a lipase activity of 7 U/ml was obtained.

References

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Citations

Sep 18, 2013·Applied Biochemistry and Biotechnology·Wei LiZhiqun Liang
Mar 7, 2014·Applied Microbiology and Biotechnology·Damien Biot-Pelletier, Vincent J J Martin
Aug 8, 2014·Applied Biochemistry and Biotechnology·Yuping ZhaoJingzhu Yang
Sep 8, 2012·Applied Biochemistry and Biotechnology·Saisubramanian Nagarajan
May 24, 2013·Applied Biochemistry and Biotechnology·Chao WangXin-Le Liang
May 10, 2013·Journal of Industrial Microbiology & Biotechnology·Ji ZhangWensheng Xiang
Feb 20, 2015·Applied Microbiology and Biotechnology·Jaejoon Jung, Woojun Park
Feb 20, 2018·Bioprocess and Biosystems Engineering·Qian-Qian TongJian-Ming Yao
Sep 25, 2020·World Journal of Microbiology & Biotechnology·Liang ChenKe Bian

Related Concepts

Diethyl sulfate, tin salt
Mima
Cold Temperature
DNA, Bacterial
Exolipase
Clastogens
Protoplasts
Thiosulfuric Acid Esters
Black Light, Ultraviolet
Genome, Bacterial

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