Genome shuffling leads to rapid phenotypic improvement in bacteria

Nature
Ying-Xin ZhangStephen B del Cardayré

Abstract

For millennia, selective breeding, on the basis of biparental mating, has led to the successful improvement of plants and animals to meet societal needs. At a molecular level, DNA shuffling mimics, yet accelerates, evolutionary processes, and allows the breeding and improvement of individual genes and subgenomic DNA fragments. We describe here whole-genome shuffling; a process that combines the advantage of multi-parental crossing allowed by DNA shuffling with the recombination of entire genomes normally associated with conventional breeding. We show that recursive genomic recombination within a population of bacteria can efficiently generate combinatorial libraries of new strains. When applied to a population of phenotypically selected bacteria, many of these new strains show marked improvements in the selected phenotype. We demonstrate the use of this approach through the rapid improvement of tylosin production from Streptomyces fradiae. This approach has the potential to facilitate cell and metabolic engineering and provide a non-recombinant alternative to the rapid production of improved organisms.

References

Mar 1, 1979·Journal of General Microbiology·D A Hopwood, H M Wright
Jan 1, 1979·American Journal of Medical Genetics·J M Opitz
Jul 14, 1977·Nature·D A HopwoodS N Cohen
Jul 4, 1978·Molecular & General Genetics : MGG·D A Hopwood, H M Wright
Jan 1, 1988·Annual Review of Microbiology·R H Baltz, E T Seno
Aug 11, 1980·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·D A Hopwood, K F Chater
Sep 1, 1981·Scientific American·Y Aharonowitz, G Cohen
Aug 1, 1981·Antimicrobial Agents and Chemotherapy·R H Baltz, E T Seno
Oct 25, 1994·Proceedings of the National Academy of Sciences of the United States of America·W P Stemmer
Jan 1, 1997·Journal of the American Academy of Child and Adolescent Psychiatry·W H SackG N Clarke
Mar 30, 1999·Nature Biotechnology·F C ChristiansW P Stemmer
Sep 3, 1999·Nature Biotechnology·J E NessJ Minshull

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Citations

Dec 4, 2004·Applied Microbiology and Biotechnology·Paolo GiudiciStefano Cassanelli
Oct 18, 2006·Applied Microbiology and Biotechnology·Hiroyuki HidaYasuhiro Yamada
May 4, 2007·Applied Microbiology and Biotechnology·Takashi YamadaYasuhiro Yamada
Mar 5, 2009·Applied Microbiology and Biotechnology·Xiyang ChenPeilin Cen
May 23, 2012·Applied Microbiology and Biotechnology·Xue-Fei WangXuan-Wei Zhou
Aug 23, 2012·Applied Microbiology and Biotechnology·Xing-An LvXin-Le Liang
Jun 23, 2009·Current Microbiology·Bheong-Uk LeeKye-Heon Oh
Jan 13, 2006·Journal of Industrial Microbiology & Biotechnology·Arnold L Demain
Jul 25, 2007·Journal of Industrial Microbiology & Biotechnology·Guo-li GongYue-zhong Li
Mar 19, 2008·Journal of Industrial Microbiology & Biotechnology·Zhi-Qiang XiongGuo-Quan Tu
Jul 24, 2010·Journal of Industrial Microbiology & Biotechnology·Dao-Qiong ZhengYu-Hua Zhao
Jan 22, 2011·Journal of Industrial Microbiology & Biotechnology·Richard H Baltz
Jan 25, 2012·Journal of Industrial Microbiology & Biotechnology·Ge JingpingPing Wenxiang
May 10, 2013·Journal of Industrial Microbiology & Biotechnology·Ji ZhangWensheng Xiang
May 17, 2013·Journal of Industrial Microbiology & Biotechnology·Pu ZhengZhihao Sun
Jan 18, 2011·Antonie van Leeuwenhoek·Mervat M A El-Gendy, Ahmed M A El-Bondkly
Jan 21, 2009·Biotechnology Letters·Lihua Hou
Jul 19, 2012·World Journal of Microbiology & Biotechnology·Xiaohong CaoLihua Hou
Jul 19, 2012·World Journal of Microbiology & Biotechnology·Pu ZhengZhi-Hao Sun
Feb 5, 2008·Science in China. Series C, Life Sciences·Kai ZhaoDongPo Zhou
Oct 13, 2009·Systems and Synthetic Biology·Manuel Porcar
Jan 10, 2009·Applied Biochemistry and Biotechnology·Z H JinP L Cen
Feb 14, 2009·Applied Biochemistry and Biotechnology·Lihua Hou
Jan 22, 2013·Applied Biochemistry and Biotechnology·Jun TanSiliang Zhang
May 24, 2013·Applied Biochemistry and Biotechnology·Chao WangXinle Liang
Dec 7, 2007·Molecular Biotechnology·Arnold L Demain, Jose L Adrio
Jan 1, 2013·The Journal of Microbiology·Jakeline Trejos JiménezMarta Novotová
Apr 29, 2005·Biomolecular Engineering·Edward G HibbertPaul A Dalby
Apr 7, 2005·Current Opinion in Chemical Biology·Takeru IshigeSakayu Shimizu
Oct 24, 2003·Trends in Biotechnology·Farid E Ahmed
Sep 27, 2002·Current Opinion in Biotechnology·Gjalt W Huisman, David Gray
Oct 29, 2003·Current Opinion in Biotechnology·Ryan T Gill
Aug 6, 2002·Trends in Microbiology·Muhamed-Kheir TahaJean-Michel Alonso

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