Genome shuffling of Clostridium acetobutylicum CICC 8012 for improved production of acetone-butanol-ethanol (ABE).

Current Microbiology
Xiaofeng GaoYanling Jin

Abstract

Genome shuffling was applied to increase ABE production of the strict anaerobe C. acetobutylicum CICC 8012. By using physical and chemical mutagenesis, strains with superior streptomycin sulfate, 2-deoxy-D-glucose and butanol tolerance levels were isolated. These strains were used for genome shuffling. The best performing strain F2-GA was screened after two rounds of genome shuffling. With 55 g glucose/l as carbon source, F2-GA produced 22.21 g ABE/l in 72 h and ABE yield reached 0.42 g/g which was about 34.53 % improvement compared to the wild type. Fermentation parameters and gene expression of several key enzymes in ABE metabolic pathways were varied significantly between F2-GA and the wild type. These results demonstrated the potential use of genome shuffling to microbial breeding which were difficult to deal with traditional methods.

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Citations

Nov 13, 2012·Applied Microbiology and Biotechnology·Kozo Ochi, Takeshi Hosaka
May 13, 2014·Applied Microbiology and Biotechnology·Tina Lütke-Eversloh
Mar 7, 2014·Applied Microbiology and Biotechnology·Damien Biot-Pelletier, Vincent J J Martin
Feb 8, 2016·Applied Microbiology and Biotechnology·H Máté de GérandoA M López-Contreras
Mar 2, 2016·World Journal of Microbiology & Biotechnology·Shu-Bo LiZong-Wen Pang
Sep 3, 2013·Biotechnology Advances·Chuang XueFeng-Wu Bai
Jan 12, 2017·Applied Microbiology and Biotechnology·Chunkai GuJianan Zhang
Jul 25, 2018·World Journal of Microbiology & Biotechnology·Qin-Geng HuangJian-Zhong Huang
May 24, 2017·Bioscience, Biotechnology, and Biochemistry·Yukinori TanakaKozo Ochi

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