Although rational method and global technique have been successfully applied in strain improvement respectively, the demand for engineering complex phenotypes required combinatorial approach. The technology of genome shuffling has been presented as a novel whole genome engineering approach for the rapid improvement of cellular phenotypes. This approach using recursive protoplast fusion with multi-parental strains offers the advantage of recombination throughout the entire genome without the necessity for genome sequence data or network information. Genome shuffling has been demonstrated as an effective method, which is not only for producing improved strain but also for providing information on complex phenotype. In this review we attempt to present the advantage of genome shuffling, introduce the procedure of this technology, summarize the applications of this approach for phenotype improvement and then give perspective on the development of this method in the future.
Nitrogen source governs the patterns of growth and pristinamycin production in 'Streptomyces pristinaespiralis'
Enhanced production of verbenol, a highly valued food flavourant, by an intergeneric fusant strain of Aspergillus niger and Penicillium digitatum
Critical variations of conjugational DNA transfer into secondary metabolite multiproducing Sorangium cellulosum strains So ce12 and So ce56: development of a mariner-based transposon mutagenesis system
Genome shuffling improves degradation of the anthropogenic pesticide pentachlorophenol by Sphingobium chlorophenolicum ATCC 39723.
Homofermentative lactate production cannot sustain anaerobic growth of engineered Saccharomyces cerevisiae: possible consequence of energy-dependent lactate export.
Heterologous production of the antifungal polyketide antibiotic soraphen A of Sorangium cellulosum So ce26 in Streptomyces lividans
Extracellular secretion of polypeptides using a modified Escherichia coli flagellar secretion apparatus
Construction of lycopene-overproducing E. coli strains by combining systematic and combinatorial gene knockout targets
Evolutionary engineering of mixed-sugar utilization by a xylose-fermenting Saccharomyces cerevisiae strain
Visualization of protoplast fusion and quantitation of recombination in fused protoplasts of auxotrophic strains of Escherichia coli
In silico aided metabolic engineering of Saccharomyces cerevisiae for improved bioethanol production
Functional expression of a P450 flavonoid hydroxylase for the biosynthesis of plant-specific hydroxylated flavonols in Escherichia coli
Yeast genes involved in response to lactic acid and acetic acid: acidic conditions caused by the organic acids in Saccharomyces cerevisiae cultures induce expression of intracellular metal metabolism genes regulated by Aft1p
Improved production of pristinamycin coupled with an adsorbent resin in fermentation by Streptomyces pristinaespiralis
Genome-shuffling improved acid tolerance and L-lactic acid volumetric productivity in Lactobacillus rhamnosus
Terahertz time-domain spectroscopy and the quantitative monitoring of mechanochemical cocrystal formation
Metabolic engineering of Escherichia coli for the production of L-valine based on transcriptome analysis and in silico gene knockout simulation
Mutation and a high-throughput screening method for improving the production of Epothilones of Sorangium
Bioproduction of p-hydroxybenzoate from renewable feedstock by solvent-tolerant Pseudomonas putida S12
Development of high-throughput screening system by single-cell reaction using microchamber array chip
Improving ethanol fermentation performance of Saccharomyces cerevisiae in very high-gravity fermentation through chemical mutagenesis and meiotic recombination
Engineering global transcription factor cyclic AMP receptor protein of Escherichia coli for improved 1-butanol tolerance
Genome shuffling of Clostridium acetobutylicum CICC 8012 for improved production of acetone-butanol-ethanol (ABE)
A genome shuffling-generated Saccharomyces cerevisiae isolate that ferments xylose and glucose to produce high levels of ethanol
Genome shuffling of Bacillus amyloliquefaciens for improving antimicrobial lipopeptide production and an analysis of relative gene expression using FQ RT-PCR
Streptomycin resistance-aided genome shuffling to improve doramectin productivity of Streptomyces avermitilis NEAU1069
Genome shuffling of marine derived bacterium Nocardia sp. ALAA 2000 for improved ayamycin production
Advances and developments in strategies to improve strains of Saccharomyces cerevisiae and processes to obtain the lignocellulosic ethanol--a review
Molecular identification using ITS sequences and genome shuffling to improve 2-deoxyglucose tolerance and xylanase activity of marine-derived fungus, Aspergillus sp. NRCF5
Enhancement of phytoremediation of Cd- and Pb-contaminated soils by self-fusion of protoplasts from endophytic fungus Mucor sp. CBRF59
Clavulanic acid production by Streptomyces clavuligerus: biogenesis, regulation and strain improvement
Improved ethanol production by a xylose-fermenting recombinant yeast strain constructed through a modified genome shuffling method
Development of a D-xylose fermenting and inhibitor tolerant industrial Saccharomyces cerevisiae strain with high performance in lignocellulose hydrolysates using metabolic and evolutionary engineering
A novel strategy to construct yeast Saccharomyces cerevisiae strains for very high gravity fermentation
Construction of novel Saccharomyces cerevisiae strains for bioethanol active dry yeast (ADY) production
The use of lactic acid-producing, malic acid-producing, or malic acid-degrading yeast strains for acidity adjustment in the wine industry
Genomic structural variations contribute to trait improvement during whole-genome shuffling of yeast
Effect of advanced intercrossing on genome structure and on the power to detect linked quantitative trait loci in a multi-parent population: a simulation study in rice
An insight into the "-omics" based engineering of streptomycetes for secondary metabolite overproduction
Saccharomyces cerevisiae: a potential host for carboxylic acid production from lignocellulosic feedstock?
Improving isopropanol tolerance and production of Clostridium beijerinckii DSM 6423 by random mutagenesis and genome shuffling
Comparative analysis of salt-tolerant gene HOG1 in a Zygosaccharomyces rouxii mutant strain and its parent strain
Characterization of cadmium-resistant endophytic fungi from Salix variegata Franch. in Three Gorges Reservoir Region, China
A comparison of whole cell directed evolution approaches in breeding of industrial strain of Saccharomyces cerevisiae
Screening and construction of Saccharomyces cerevisiae strains with improved multi-tolerance and bioethanol fermentation performance
Molecular, Physiological and Phenotypic Characterization of Paracoccus denitrificans ATCC 19367 Mutant Strain P-87 Producing Improved Coenzyme Q10
Intraspecific protoplast fusion of Brettanomyces anomalus for improved production of an extracellular β-glucosidase
Genome shuffling of Saccharomyces cerevisiae for enhanced glutathione yield and relative gene expression analysis using fluorescent quantitation reverse transcription polymerase chain reaction
ARTP mutation and genome shuffling of ABE fermentation symbiotic system for improvement of butanol production
Endophytic fungal communities of Polygonum acuminatum and Aeschynomene fluminensis are influenced by soil mercury contamination
Genome shuffling and ribosome engineering of Streptomyces virginiae for improved virginiamycin production
A new approach for breeding low-temperature-resistant Volvariella volvacea strains: Genome shuffling in edible fungi
Genome shuffling of Lactococcus lactis subspecies lactis YF11 for improving nisin Z production and comparative analysis
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