Genome shuffling: Progress and applications for phenotype improvement

Biotechnology Advances
Jixian GongXueming Zhao


Although rational method and global technique have been successfully applied in strain improvement respectively, the demand for engineering complex phenotypes required combinatorial approach. The technology of genome shuffling has been presented as a novel whole genome engineering approach for the rapid improvement of cellular phenotypes. This approach using recursive protoplast fusion with multi-parental strains offers the advantage of recombination throughout the entire genome without the necessity for genome sequence data or network information. Genome shuffling has been demonstrated as an effective method, which is not only for producing improved strain but also for providing information on complex phenotype. In this review we attempt to present the advantage of genome shuffling, introduce the procedure of this technology, summarize the applications of this approach for phenotype improvement and then give perspective on the development of this method in the future.


Mar 1, 1979·Journal of General Microbiology·D A Hopwood, H M Wright
Jun 21, 1991·Science·Gregory Stephanopoulos, J J Vallino
Jun 21, 1991·Science·J E Bailey
Aug 4, 1994·Nature·Willem P C Stemmer
Jan 1, 1996·Trends in Biotechnology·C KatoK Horikoshi
May 1, 1997·Nature Biotechnology·A CrameriWillem P C Stemmer
Jul 6, 2000·Bioscience, Biotechnology, and Biochemistry·C HansawasdiT Kasai
Feb 8, 2002·Nature·Ying-Xin ZhangStephen del Cardayré
Jun 29, 2002·Nature Biotechnology·Gregory Stephanopoulos
Jun 29, 2002·Nature Biotechnology·Ranjan PatnaikStephen del Cardayré
Apr 5, 2003·Applied and Environmental Microbiology·Marco Sonderegger, Uwe Sauer
Jun 5, 2003·Nature Biotechnology·Vincent J J MartinJay D Keasling
Oct 9, 2003·Applied and Environmental Microbiology·Bryan Julien, Ruby Fehd
Jan 1, 1983·Biotechnology Advances·S Scheinbach
Apr 5, 2005·Nature Biotechnology·Katariina MajanderBenita Westerlund-Wikström
Jun 1, 2005·Trends in Biotechnology·Sang Yup LeeTae Yong Kim
Aug 24, 2005·Bioscience, Biotechnology, and Biochemistry·Hiroyuki HidaYasuhiro Yamada
Sep 10, 2005·Applied and Environmental Microbiology·Yajun YanMattheos A G Koffas
Dec 8, 2005·Applied and Environmental Microbiology·Nick J P WierckxJan Wery
Feb 24, 2006·Journal of Industrial Microbiology & Biotechnology·Helge B Bode, Rolf Müller
Jul 18, 2006·Nature Biotechnology·Brian F PflegerJay D Keasling
Aug 24, 2006·Bioscience, Biotechnology, and Biochemistry·Hiroyuki HidaYasuhiro Yamada
Oct 18, 2006·Applied Microbiology and Biotechnology·Hiroyuki HidaYasuhiro Yamada
Apr 28, 2007·Proceedings of the National Academy of Sciences of the United States of America·Jin Hwan ParkSang Yup Lee
Jul 17, 2007·Biotechnology and Applied Biochemistry·Pingying WeiNing Jiang
Jul 25, 2007·Journal of Industrial Microbiology & Biotechnology·Guo-li GongYue-zhong Li
Sep 8, 2007·Sheng wu gong cheng xue bao = Chinese journal of biotechnology·Jun LinMing-Zi Wang
Oct 30, 2007·Journal of Bioscience and Bioengineering·Takeshi FukudaMitsuyoshi Ueda
Dec 7, 2007·Molecular Systems Biology·Kwang Ho LeeSang Yup Lee
Feb 5, 2008·Science in China. Series C, Life Sciences·Kai ZhaoDongPo Zhou


Apr 3, 2012·Applied Microbiology and Biotechnology·Hongfang ZhangRongrong Jiang
Jan 25, 2012·Journal of Industrial Microbiology & Biotechnology·Ge JingpingPing Wenxiang
May 10, 2013·Journal of Industrial Microbiology & Biotechnology·Ji ZhangWensheng Xiang
May 17, 2013·Journal of Industrial Microbiology & Biotechnology·Pu ZhengZhihao Sun
Jan 18, 2011·Antonie van Leeuwenhoek·Mervat M A El-Gendy, Ahmed M A El-Bondkly
Jul 19, 2012·World Journal of Microbiology & Biotechnology·Pu ZhengZhi-Hao Sun
Sep 18, 2013·Applied Biochemistry and Biotechnology·Wei LiZhiqun Liang
Dec 19, 2013·Applied Microbiology and Biotechnology·Dao-Qiong ZhengXue-Chang Wu
Oct 1, 2013·BioMed Research International·Amit Kumar ChaudharyJae Kyung Sohng
Aug 8, 2014·Applied Biochemistry and Biotechnology·Yuping ZhaoJingzhu Yang
Jun 28, 2014·Applied Microbiology and Biotechnology·Anders G SandströmMarie F Gorwa-Grauslund
Aug 15, 2014·Applied Biochemistry and Biotechnology·Pornchanok ChalopagornKiattawee Choowongkomon
Feb 8, 2016·Applied Microbiology and Biotechnology·H Máté de GérandoA M López-Contreras
May 20, 2011·Current Opinion in Biotechnology·Lauren B A Woodruff, Ryan T Gill
Mar 2, 2016·World Journal of Microbiology & Biotechnology·Shu-Bo LiZong-Wen Pang
May 23, 2013·Journal of the Science of Food and Agriculture·Yonghua WeiLi-Hua Hou
Jan 8, 2014·Microbial Biotechnology·Jun ShiHuiping Deng
Apr 15, 2014·FEMS Microbiology Reviews·Jan SteenselsKevin J Verstrepen
Jul 7, 2011·Biotechnology Advances·Lei PeiWei Wei
Jun 5, 2015·World Journal of Microbiology & Biotechnology·Seema PatelJ Satya Eswari
Dec 3, 2014·Trends in Biotechnology·Bikram P UpadhyayaLew P Christopher
Jan 31, 2015·Applied Microbiology and Biotechnology·Wenshan Liu, Rongrong Jiang
Nov 18, 2016·Biotechnology Letters·Shenghai WangYi Zhang
Jan 12, 2017·Applied Microbiology and Biotechnology·Chunkai GuJianan Zhang
Feb 20, 2018·Bioprocess and Biosystems Engineering·Qian-Qian TongJian-Ming Yao
Apr 14, 2016·Natural Product Reports·Mingzi M ZhangHuimin Zhao

Related Concepts

Related Feeds

Biofuels (ASM)

Biofuels are produced through contemporary processes from biomass rather than geological processes involved in fossil fuel formation. Examples include biodiesel, green diesel, biogas, etc. Discover the latest research on biofuels in this feed.

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.