Genome size and structure determine efficiency of postinternalization steps and gene transfer of capsid-modified adenovirus vectors in a cell-type-specific manner

Journal of Virology
Dmitry M ShayakhmetovAndré Lieber

Abstract

Adenovirus serotype 5 (Ad5) vectors containing Ad B-group fibers have become increasingly popular as gene transfer vectors because they efficiently transduce human cell types that are relatively refractory to Ad5 infection. So far, most B-group fiber-containing vectors have been first-generation vectors, deleted of E1 and/or E3 genes. Transduction with these vectors, however, results in viral gene expression and is associated with cytotoxicity and immune responses against transduced cells. To circumvent these problems, we developed fiber-chimeric Ad vectors devoid of all viral genes that were produced either by the homologous recombination of first-generation vectors or by using the Cre/lox-based helper virus system. In this study we compared early steps of infection between first-generation (35-kb genome) and Ad vectors devoid of all viral genes with genome sizes of 28 kb and 12.6 kb. All vectors possessed an Ad35-derived fiber knob domain, which uses CD46 as a primary attachment receptor. Using immortalized human hematopoietic cell lines and primary human CD34-positive hematopoietic cells, we found that the Ad genome size did not affect the efficiency of virus attachment to and internalization into cells. Furthermore, indepen...Continue Reading

References

Nov 1, 1992·FEMS Microbiology Letters·E EverittY Blixt
May 11, 1989·Nucleic Acids Research·M L Wong, M T Hsu
Sep 5, 1985·Journal of Molecular Biology·R M BurnettJ L White
Nov 1, 1985·Journal of Virology·J van Oostrum, R M Burnett
Nov 1, 1969·Virology·W G LaverH G Pereira
Oct 6, 1997·The EMBO Journal·U F GreberA Helenius
Feb 3, 2000·Proceedings of the National Academy of Sciences of the United States of America·V SandigC T Caskey
Feb 23, 2000·Journal of Virology·D M ShayakhmetovA Lieber
Oct 24, 2000·Journal of Virology·D M Shayakhmetov, A Lieber
Nov 20, 2001·Stem Cells·C MantelH E Broxmeyer
Mar 9, 2002·Methods in Enzymology·Cheryl A CarlsonAndré Lieber
Jul 4, 2002·Molecular Therapy : the Journal of the American Society of Gene Therapy·Cheryl A CarlsonAndré Lieber
Jul 4, 2002·Molecular Therapy : the Journal of the American Society of Gene Therapy·Cheryl A CarlsonAndré Lieber
Jun 11, 2003·The Journal of Gene Medicine·Oliver Meier, Urs F Greber
Aug 9, 2003·Current Biology : CB·Alexander DammermannKaren Oegema
Aug 14, 2003·Journal of Virology·Anna SegermanNiklas Arnberg
Oct 21, 2003·Nature Medicine·Anuj GaggarAndré Lieber
Apr 14, 2004·Journal of Virology·Dominique SirenaSilvio Hemmi

❮ Previous
Next ❯

Citations

Feb 8, 2006·Molecular Therapy : the Journal of the American Society of Gene Therapy·Nelson DiPaoloAndré Lieber
Oct 1, 2009·Molecular Therapy : the Journal of the American Society of Gene Therapy·Michael A Kennedy, Robin J Parks
Jan 28, 2006·Cancer Gene Therapy·R G van der MostD J Nelson
Aug 23, 2007·Molecular Therapy : the Journal of the American Society of Gene Therapy·Sophie RogéeMorvane Colin
Sep 5, 2008·Proceedings of the National Academy of Sciences of the United States of America·Keiichiro SuzukiKohnosuke Mitani
Aug 30, 2008·Journal of Virology·Hongjie WangAndré Lieber
Nov 28, 2008·Journal of Virology·Adam C SmithRobin J Parks
Sep 22, 2005·Proceedings of the National Academy of Sciences of the United States of America·Fumi OhbayashiKohnosuke Mitani
Jul 8, 2014·Nature Communications·Guang-Hui LiuJuan Carlos Izpisua Belmonte
Jul 6, 2014·Gene Therapy·A PuppoA Auricchio
Oct 28, 2009·Expert Opinion on Biological Therapy·Jin XuDeliang Fu
May 21, 2011·Cell Stem Cell·Guang-Hui LiuJuan Carlos Izpisua Belmonte
Jun 1, 2006·Molecular Therapy : the Journal of the American Society of Gene Therapy·Laurent MaillyMorvane Colin
Jul 23, 2020·Molecular Therapy. Methods & Clinical Development·Jian GaoAnja Ehrhardt

❮ Previous
Next ❯

Related Concepts

Related Feeds

Alzheimer's Disease: Endosomes

Dysfunctional endosomal trafficking may be associated with Alzheimer’s disease (AD) pathology. Targeting the endosome may advance treatment options for AD. Here is the latest research on endosomes and AD.