PMID: 9549065Apr 29, 1998Paper

Genome-specific repetitive DNA and RAPD markers for genome identification in Elymus and Hordelymus

Genome Génome / Conseil National De Recherches Canada
S SvitashevR R Wang

Abstract

We have developed RFLP and RAPD markers specific for the genomes involved in the evolution of Elymus species, i.e., the St, Y, H, P, and W genomes. Two P genome specific repetitive DNA sequences, pAgc1 (350 bp) and pAgc30 (458 bp), and three W genome specific sequences, pAuv3 (221 bp), pAuv7 (200 bp), and pAuv13 (207 bp), were isolated from the genomes of Agropyron cristatum and Australopyrum velutinum, respectively. Attempts to find Y genome specific sequences were not successful. Primary-structure analysis demonstrated that pAgc1 (P genome) and pAgc30 (P genome) share 81% similarity over a 227-bp stretch. The three W genome specific sequences were also highly homologous. Sequence comparison analysis revealed no homology to sequences in the EMBL-GenBank databases. Three to four genome-specific RAPD markers were found for each of the five genomes. Genome-specific bands were cloned and demonstrated to be mainly low-copy sequences present in various Triticeae species. The RFLP and RAPD markers obtained, together with the previously described H and St genome specific clones pHch2 and pP1Taq2.5 and the Ns genome specific RAPD markers were used to investigate the genomic composition of a few Elymus species and Hordelymus europaeus, ...Continue Reading

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Citations

Sep 12, 2001·Genome Génome / Conseil National De Recherches Canada·P BoganiM Buiatti
Sep 12, 2001·Genome Génome / Conseil National De Recherches Canada·R GuadagnuoloF Felber
Mar 23, 2002·Genome Génome / Conseil National De Recherches Canada·K K NkongoloW S Gratton
Mar 23, 2002·Genome Génome / Conseil National De Recherches Canada·Jong-Min KoHuhn-Pal Moon
Dec 28, 2002·Genome Génome / Conseil National De Recherches Canada·Roberta J Mason-GamerClaire M Anderson
Apr 2, 2005·Biotechnology Progress·G Di BernardoA Cascino
Jul 19, 2011·Plant Science : an International Journal of Experimental Plant Biology·Shao-An FangMei-Chu Chung
Mar 17, 2007·Hereditas·Pernilla Ellneskog-StaamRoland von Bothmer

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