PMID: 25762640Mar 13, 2015Paper

Genome-wide 5-hydroxymethylcytosine modification pattern is a novel epigenetic feature of globozoospermia

Oncotarget
Xiu-Xia WangDa Li

Abstract

Discovery of 5-hydroxymethylcytosine (5hmC) in mammalian genomes has excited the field of epigenetics, but information on the genome-wide distribution of 5hmC is limited. Globozoospermia is a rare but severe cause of male infertility. To date, the epigenetic mechanism, especially 5hmC profiles involved in globozoospermia progression, remains largely unknown. Here, utilizing the chemical labeling and biotin-enrichment approach followed by Illumina HiSeq sequencing, we showed that (i) 6664, 9029 and 6318 genes contain 5hmC in normal, abnormal, and globozoospermia sperm, respectively; (ii) some 5hmC-containing genes significantly involves in spermatogenesis, sperm motility and morphology, and gamete generation; (iii) 5hmC is exclusively localized in sperm intron; (iv) approximately 40% imprinted genes have 5hmC modification in sperm genomes, but globozoospermia sperm exhibiting a large portion of imprinted genes lose the 5hmC modification; (v) six imprinted genes showed different 5hmC patterns in abnormal sperm (GDAP1L1, GNAS, KCNK9, LIN28B, RB1, RTL1), and five imprinted genes showed different 5hmC patterns in globozoospermia sperm (KCNK9, LIN28B, RB1, SLC22A18, ZDBF2). These results suggested that differences in genome-wide 5hmC...Continue Reading

References

Mar 5, 2005·Human Reproduction·Olivier PirrelloStéphane Viville
Sep 30, 2006·Human Reproduction Update·A H D M DamJ A M Kremer
Sep 19, 2008·Genome Biology·Yong ZhangX Shirley Liu
Jan 10, 2009·Nature Protocols·Da Wei HuangRichard A Lempicki
May 20, 2009·Bioinformatics·Heng Li, Richard Durbin
Dec 15, 2010·Bioinformatics·Michael E SmootTrey Ideker
May 10, 2011·Nature·William A PastorAnjana Rao
Mar 1, 2012·Gene Expression Patterns : GEP·Kan-ichiro IharaKatsuhiko Nishimori
Jun 14, 2013·Nature Communications·Haiyun GanFuchou Tang
Aug 24, 2013·Proceedings of the National Academy of Sciences of the United States of America·Bradley M ColquittStavros Lomvardas
Nov 21, 2013·Cancer Biology & Therapy·Chao Ye, Lan Li

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