Genome-wide analysis of androgen receptor binding and gene regulation in two CWR22-derived prostate cancer cell lines.

Endocrine-related Cancer
Honglin ChenMaria Mudryj

Abstract

Prostate carcinoma (CaP) is a heterogeneous multifocal disease where gene expression and regulation are altered not only with disease progression but also between metastatic lesions. The androgen receptor (AR) regulates the growth of metastatic CaPs; however, sensitivity to androgen ablation is short lived, yielding to emergence of castrate-resistant CaP (CRCaP). CRCaP prostate cancers continue to express the AR, a pivotal prostate regulator, but it is not known whether the AR targets similar or different genes in different castrate-resistant cells. In this study, we investigated AR binding and AR-dependent transcription in two related castrate-resistant cell lines derived from androgen-dependent CWR22-relapsed tumors: CWR22Rv1 (Rv1) and CWR-R1 (R1). Expression microarray analysis revealed that R1 and Rv1 cells had significantly different gene expression profiles individually and in response to androgen. In contrast, AR chromatin immunoprecipitation (ChIP) combined with promoter DNA microarrays (ChIP-on-chip) studies showed that they have a similar AR-binding profile. Coupling of the microarray study with ChIP-on-chip analysis identified direct AR targets. The most prominent function of transcripts that were direct AR targets w...Continue Reading

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Citations

Jul 19, 2013·Hormones & Cancer·Maria Mudryj, Clifford G Tepper
Feb 26, 2013·Nucleic Acids Research·Kelly CoffeyCraig N Robson
Oct 25, 2013·International Journal of Biological Sciences·Kuppusamy Balamurugan, Esta Sterneck
Oct 30, 2014·Journal of Molecular Endocrinology·Ye ZhouJeremy O Jones
Apr 19, 2011·International Journal of Cancer. Journal International Du Cancer·Dennis W WolffYaping Tu
Sep 14, 2013·Carcinogenesis·Diego Iglesias-GatoAmilcar Flores-Morales
Mar 12, 2015·Endocrine-related Cancer·Rosalinda M SavoyParamita M Ghosh
Sep 9, 2016·Endocrine-related Cancer·Paramita M Ghosh
Feb 24, 2018·The Prostate·Ben T CopelandJeremy O Jones
Nov 20, 2016·Molecular Cancer Research : MCR·Neha G SabnisWendy J Huss
Nov 11, 2020·International Journal of Molecular Sciences·Alicja KamińskaAnna Hejmej

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