Genome-wide analysis of Chongqing native intersexual goats using next-generation sequencing

3 Biotech
E Guang-XinYong-Fu Huang

Abstract

Sex reversal has been studied extensively in vertebrate species, particularly in domestic goats, because polled intersex syndrome (PIS) has seriously affected their production efficiency. In the present study, we used histopathologically diagnosed cases of PIS to identify correlated genomic regions and variants using representative selection signatures and performed GWAS using Restriction-Site Associated Resequencing DNA. We identified 171 single-nucleotide polymorphisms (SNPs) that may have contributed to this phenotype, and 53 SNPs were determined to be located in coding regions using a general linear model. The transcriptome data sets of differentially expressed genes (DEGs) in the pituitary tissues of intersexual and nonintersexual goats were examined using high-throughput technology. A total of 10,063 DEGs and 337 long noncoding RNAs were identified. The DEGs were clustered into 56 GO categories and determined to be significantly enriched in 53 signaling pathways by KEGG analysis. In addition, according to qPCR results, PSPO2 and FSH were significantly more highly expressed in sexually mature pituitary tissues of intersexual goats compared to healthy controls (nonintersexual). These results demonstrate that certain novel p...Continue Reading

References

May 1, 1990·Andrologia·C Vallenzasca, A Galli
Jan 1, 1982·Cytogenetics and Cell Genetics·T A BongsoT K Mukherjee
Mar 1, 1994·Journal of Reproduction and Fertility·E PailhouxC Cotinot
Dec 1, 2001·Nature Genetics·E PailhouxD Vaiman
Dec 19, 2001·The Journal of Experimental Zoology·E PailhouxC Cotinot
May 2, 2002·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Eric PailhouxCorinne Cotinot
Jun 26, 2007·Bioinformatics·Peter J BradburyEdward S Buckler
May 20, 2009·Bioinformatics·Heng Li, Richard Durbin
Feb 4, 2014·Current Biology : CB·Laurent BoulangerEric Pailhoux
Jun 28, 2015·Biochimica Et Biophysica Acta·Marion LapierreVincent Cavaillès
Dec 3, 2015·European Archives of Psychiatry and Clinical Neuroscience·Niki AntypaAlessandro Serretti
Oct 21, 2016·PloS One·Suély V SilvaVanessa M Freitas
May 20, 2017·Advances in Experimental Medicine and Biology·Rotem MaozHermona Soreq
Jun 19, 2017·Journal of Assisted Reproduction and Genetics·L TamburinoA E Calogero
Jul 5, 2017·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Satoshi HirohataTakashi Ohtsuki
Apr 24, 2018·DNA and Cell Biology·Gustavo Mendonça AndréCaio Parente Barbosa
May 8, 2018·Proceedings of the National Academy of Sciences of the United States of America·Abigail HarrisAndy Greenfield
Jun 16, 2018·Science·Nitzan GonenRobin Lovell-Badge

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.