DOI: 10.1101/486746Dec 4, 2018Paper

Genome-wide analysis of drug resistant Mycobacterium tuberculosis isolates causing pulmonary and extrapulmonary tuberculosis in Russia

BioRxiv : the Preprint Server for Biology
E ChernyaevaStephen J O'Brien


Mycobacterium tuberculosisis a highly studied pathogen due to public health importance. Despite progress in M.tuberculosis genome diversity analysis, there remain insufficient data on genome analysis of M.tuberculosis strains associated with pulmonary vs. extrapulmonary TB (PTB or XPTB respectively) tissue localization. Here we conduct comparative analysis of whole-genome sequence (WGS) for clinical M.tuberculosis strains collected from patients with PTB (n=72) and XPTB (n=73) localization. We further analyze the incidence of point mutations widely used for drug resistance detection in laboratory practice. M.tuberculosis isolates were collected from patients with varying clinical status from 2007 to 2014 in Russia. Bacterial DNA was extracted and sequenced using MiSeq platform (Illumina). WGSdata allowed identifying M.tuberculosis substrains associated with distinctions in the occurrence in PTB v.s. XPTB cases. There occurred little statistically significant specific DNA variants or genotypes diagnostic of tissue distribution, however phylogenetic analyses did reveal M.tuberculosis genetic substrains associated with TB localization. XPTB was associated with Beijing CAO, A and 4.8 groups, while PTB localization was associated wi...Continue Reading

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