Genome-wide analysis of laccase genes in moso bamboo highlights PeLAC10 involved in lignin biosynthesis and in response to abiotic stresses

Plant Cell Reports
Lichao LiZhimin Gao

Abstract

Twenty-three PeLACs have been identified in moso bamboo, overexpression of PeLAC10 increases the lignin content and confers drought and phenolic acid tolerance in transgenic Arabidopsis. Laccases (LACs) have multifunction involved in the processes of cell elongation, lignification and stress response in plants. However, the function of laccases in bamboo remain unclear. Here, a total of 23 laccase genes (PeLAC1-PeLAC23) were identified in moso bamboo (Phyllostachys edulis). The diverse gene structure and expression pattern of PeLACs suggested that their function should be spatiotemporal and complicated, which was supported by the expression profiles in different tissues of moso bamboo. Eighteen PeLACs were identified as the targets of ped-miR397. The putative ped-miR397-binding site in the coding region of PeLAC10 was further confirmed by RLM-5' RACE, indicating that PeLAC10 was regulated by ped-miR397 after transcription. With the increasing shoot height, the expression abundance of PeLAC10 was up-regulated and reached the maximum in 15 cm shoots, while that of ped-miR397 was relative lower and showed the minimum in 15 cm shoots. PeLAC10 was up-regulated obviously under both ABA (100 μmol L-1) and NaCl (400 mmol L-1) treatment...Continue Reading

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Citations

Dec 18, 2020·Functional Plant Biology : FPB·Shili HuangYulin Tang

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Datasets Mentioned

BETA
ERP001341

Methods Mentioned

BETA
PCR
transgenic
electrophoresis
MDA
RNA-Seq

Software Mentioned

Gene Structure Display Server
MEGA
GenScript Real - time PCR Primer Design
BioEdit
PlantCARE
MEGA6
psRNATarget
tBLASTn

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