Genome-wide analysis of longevity in nutrient-deprived Saccharomyces cerevisiae reveals importance of recycling in maintaining cell viability.

Environmental Microbiology
H M DaveyGareth W Griffith

Abstract

Although typically cosseted in the laboratory with constant temperatures and plentiful nutrients, microbes are frequently exposed to much more stressful conditions in their natural environments where survival and competitive fitness depend upon both growth rate when conditions are favourable and on persistence in a viable and recoverable state when they are not. In order to determine the role of genetic heterogeneity in environmental fitness we present a novel approach that combines the power of fluorescence-activated cell sorting with barcode microarray analysis and apply this to determining the importance of every gene in the Saccharomyces cerevisiae genome in a high-throughput, genome-wide fitness screen. We have grown > 6000 heterozygous mutants together and exposed them to a starvation stress before using fluorescence-activated cell sorting to identify and isolate those individual cells that have not survived the stress applied. Barcode array analysis of the sorted and total populations reveals the importance of cellular recycling mechanisms (autophagy, pexophagy and ribosome breakdown) in maintaining cell viability during starvation and provides compelling evidence for an important role for fatty acid degradation in maint...Continue Reading

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Citations

Jun 14, 2014·Database : the Journal of Biological Databases and Curation·Wen WeiFeng-Biao Guo
Jan 7, 2016·Autophagy·Brice E FloydDiane C Bassham
Apr 25, 2013·Biological Chemistry·Ülo MaiväliTanel Tenson
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Jan 24, 2018·Journal of Cell Science·Gaurav BarveRavi Manjithaya
Sep 30, 2020·The Journal of Microbiology·Kwang-Woo JungYong-Sun Bahn

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