Genome-wide analysis of RopGEF gene family to identify genes contributing to pollen tube growth in rice (Oryza sativa)

BMC Plant Biology
Eui-Jung KimYu-Jin Kim

Abstract

In plants, the key roles played by RopGEF-mediated ROP signaling in diverse processes, including polar tip growth, have been identified. Despite their important roles in reproduction, a comprehensive analysis of RopGEF members has not yet been performed in rice (Oryza sativa). To determine whether RopGEF regulators are involved in rice pollen tube growth, we performed genome-wide analysis of this family in rice. Phylogenomic and meta-expression analysis of eleven RopGEFs in rice showed that four genes were preferentially expressed in mature pollen. These four genes contain the plant-specific Rop nucleotide exchanger (PRONE) domain and possible phosphorylated residues, suggesting a conserved role in polar tip growth with Arabidopsis thaliana. In subcellular localization analysis of the four RopGEFs through tobacco (Nicotiana benthamiana) infiltration, four proteins were predominantly identified in plasma membrane. Moreover, double mutants of RopGEF2/8 exhibited reduced pollen germination, causing partial male sterility. These genes possess unique cis-acting elements in their promoters compared with the other RopGEF genes. In this study, four RopGEF genes were identified as pollen-specific gene in eleven members of rice, and the ...Continue Reading

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Citations

Jul 12, 2020·The Plant Journal : for Cell and Molecular Biology·Woo-Jong HongKi-Hong Jung
Dec 22, 2020·The Plant Journal : for Cell and Molecular Biology·Yu-Jin KimKi-Hong Jung
Jun 19, 2021·The Plant Journal : for Cell and Molecular Biology·Yu-Jin KimKi-Hong Jung
May 29, 2021·Biological Reviews of the Cambridge Philosophical Society·Mohammad ArefianT S Keshava Prasad
Sep 15, 2021·Development, Growth & Differentiation·Yangfan XuWanqi Liang

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Methods Mentioned

BETA
GTPases
nucleotide exchange
nucleotide exchanger
PCR
fluorescence microscopy
transgenic

Software Mentioned

Affy package
MODEL Workspace
Multiple Em for Motif Elicitation ( MEME ) suite
SWISS
MEME
TOMTOM
Chimera
R
ClustalW
MEGA

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