Genome-Wide Analysis of the Fasciclin-Like Arabinogalactan Protein Gene Family Reveals Differential Expression Patterns, Localization, and Salt Stress Response in Populus

Frontiers in Plant Science
Lina ZangXiaohua Su

Abstract

Fasciclin-like arabinogalactan proteins (FLAs) are a subclass of arabinogalactan proteins (AGPs) involved in plant growth, development and response to abiotic stress. Although many studies have been performed to identify molecular functions of individual family members, little information is available on genome-wide identification and characterization of FLAs in the genus Populus. Based on genome-wide analysis, we have identified 35 Populus FLAs which were distributed on 16 chromosomes and phylogenetically clustered into four major groups. Gene structure and motif composition were relatively conserved in each group. All the members contained N-terminal signal peptide, 23 of which included predicted glycosylphosphatidylinositol (GPI) modification sites and were anchored to plasma membranes. Subcellular localization analysis showed that PtrFLA2/20/26 were localized in cell membrane and cytoplasm of protoplasts from Populus stem-differentiating xylem. The Ka/Ks ratios showed that purifying selection has played a leading role in the long-term evolutionary period which greatly maintained the function of this family. The expression profiles showed that 32 PtrFLAs were differentially expressed in four tissues at four seasons based on ...Continue Reading

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Citations

Oct 8, 2016·Cell·Caren ChangElliot M Meyerowitz
May 28, 2019·International Journal of Molecular Sciences·Artur PinskiRobert Hasterok
Nov 30, 2019·Frontiers in Plant Science·Katarzyna Marzec-SchmidtAgnieszka Bagniewska-Zadworna
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Mar 12, 2021·Plant Science : an International Journal of Experimental Plant Biology·Hsin-Hung LinYu-Chi Chen
Jun 4, 2021·Frontiers in Plant Science·Dagmar HromadováEdita Tylová
Jul 10, 2021·Plant & Cell Physiology·Daisuke TakahashiDavid P Livingston Iii
Oct 12, 2021·Frontiers in Plant Science·Pedro Augusto Medeiros BarbosaTerence Luke Molnar

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Datasets Mentioned

BETA
GSE56023

Methods Mentioned

BETA
transgenic
PCR
transfection
Confocal Microscopy
glycosylation

Software Mentioned

Multiple Em for Motif Elucidation ( MEME )
SMART
big
MEGA
HMMER3
Clustal X
Plant
Probe Match
MEME
MEGA5

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