Genome-wide analysis of the maternal-to-zygotic transition in Drosophila primordial germ cells.

Genome Biology
Najeeb U SiddiquiHoward D Lipshitz

Abstract

During the maternal-to-zygotic transition (MZT) vast changes in the embryonic transcriptome are produced by a combination of two processes: elimination of maternally provided mRNAs and synthesis of new transcripts from the zygotic genome. Previous genome-wide analyses of the MZT have been restricted to whole embryos. Here we report the first such analysis for primordial germ cells (PGCs), the progenitors of the germ-line stem cells. We purified PGCs from Drosophila embryos, defined their proteome and transcriptome, and assessed the content, scale and dynamics of their MZT. Transcripts encoding proteins that implement particular types of biological functions group into nine distinct expression profiles, reflecting coordinate control at the transcriptional and posttranscriptional levels. mRNAs encoding germ-plasm components and cell-cell signaling molecules are rapidly degraded while new transcription produces mRNAs encoding the core transcriptional and protein synthetic machineries. The RNA-binding protein Smaug is essential for the PGC MZT, clearing transcripts encoding proteins that regulate stem cell behavior, transcriptional and posttranscriptional processes. Computational analyses suggest that Smaug and AU-rich element bind...Continue Reading

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Citations

Jul 3, 2013·Psychological Medicine·N AntypaA Serretti
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Datasets Mentioned

BETA
GSE34397

Methods Mentioned

BETA
flow cytometry
fluorescence activated cell sorting
confocal microscopy
FACS

Software Mentioned

Proteome
PicTar
Scaffold
SAM
Sequest [UNK]
GeneMANIA
TargetScanFly
Targetscan
MuDPIT
ArrayStar

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