Genome-Wide Assessment of DNA Methylation in Chicken Cardiac Tissue Exposed to Different Incubation Temperatures and CO2 Levels

Frontiers in Genetics
Ryan J CorbettOle Madsen

Abstract

Temperature and CO2 concentration during incubation have profound effects on broiler chick development, and numerous studies have identified significant effects on hatch heart weight (HW) as a result of differences in these parameters. Early life environment has also been shown to affect broiler performance later in life; it has thus been suggested that epigenetic mechanisms may mediate long-term physiological changes induced by environmental stimuli. DNA methylation is an epigenetic modification that can confer heritable changes in gene expression. Using reduced-representation bisulfite sequencing (RRBS), we assessed DNA methylation patterns in cardiac tissue of 84 broiler hatchlings incubated at two egg shell temperatures (EST; 37.8°C and 38.9°C) and three CO2 concentrations (0.1%, 0.4%, and 0.8%) from day 8 of incubation onward. We assessed differential methylation between EST treatments and identified 2,175 differentially methylated (DM) CpGs (1,121 hypermethylated, 1,054 hypomethylated at 38.9° vs. 37.8°) in 269 gene promoters and 949 intragenic regions. DM genes (DMGs) were associated with heart developmental processes, including cardiomyocyte proliferation and differentiation. We identified enriched binding motifs among ...Continue Reading

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Citations

Jun 1, 2021·Poultry Science·Marek BednarczykEwa Grochowska
Jul 10, 2021·Genome Génome / Conseil National De Recherches Canada·Kai WangGuoqing Tang

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Datasets Mentioned

BETA
PRJEB39999

Methods Mentioned

BETA
RRBS
PCR
reverse-transcription PCR
GTPase

Software Mentioned

methylKit
ENCODE
GSEA
seeker2
Trim Galore
bcl2fastq
bs
Fastq
MEME
Analysis of Motif Enrichment ( AME )

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