Genome-wide assessment of post-transcriptional control in the fly brain

Frontiers in Molecular Neuroscience
Shaul MezanSebastian Kadener

Abstract

Post-transcriptional control of gene expression has central importance during development and adulthood and in physiology in general. However, little is known about the extent of post-transcriptional control of gene expression in the brain. Most post-transcriptional regulatory effectors (e.g., miRNAs) destabilize target mRNAs by shortening their polyA tails. Hence, the fraction of a given mRNA that it is fully polyadenylated should correlate with its stability and serves as a good measure of post-transcriptional control. Here, we compared RNA-seq datasets from fly brains that were generated either from total (rRNA-depleted) or polyA-selected RNA. By doing this comparison we were able to compute a coefficient that measures the extent of post-transcriptional control for each brain-expressed mRNA. In agreement with current knowledge, we found that mRNAs encoding ribosomal proteins, metabolic enzymes, and housekeeping genes are among the transcripts with least post-transcriptional control, whereas mRNAs that are known to be highly unstable, like circadian mRNAs and mRNAs expressing synaptic proteins and proteins with neuronal functions, are under strong post-transcriptional control. Surprisingly, the latter group included many spec...Continue Reading

Citations

Jul 16, 2014·Frontiers in Molecular Neuroscience·Hermona Soreq
Feb 10, 2018·Wiley Interdisciplinary Reviews. RNA·Manon TorresAnne-Marie François-Bellan
Mar 6, 2019·Proceedings of the National Academy of Sciences of the United States of America·Brigitte GrimaFrançois Rouyer
Aug 13, 2017·Genetics·Esteban J BeckwithMarcelo J Yanovsky

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Datasets Mentioned

BETA
GSE29972

Methods Mentioned

BETA
RNA-seq
chips
dissection

Software Mentioned

Target
Scan
DAVID
R
TargetScanFly
Affymetrix
gplots
Affymetrix Expression ConsoleTM

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