Genome-wide association studies for inflorescence type and remontancy in Hydrangea macrophylla.

Horticulture Research
Xingbo Wu, Lisa W Alexander

Abstract

Inflorescence type and remontancy are two valuable traits in bigleaf hydrangea (Hydrangea macrophylla L.) and both are recessively inherited. Molecular marker-assisted selection (MAS) can greatly reduce the time necessary to breed cultivars with desired traits. In this study, a genome-wide association study (GWAS) using 5803 single-nucleotide polymorphisms (SNPs) was performed using a panel of 82 bigleaf hydrangea cultivars. One SNP locus (Hy_CAPS_Inflo) associated with inflorescence type was identified with general linear model (GLM) and mixed linear model (MLM) methods that explained 65.5% and 36.1% of the phenotypic variations, respectively. Twenty-three SNPs associated with remontancy were detected in GLM whereas no SNP was detected in MLM. The SNP locus (Hy_CAPS_Inflo) was converted to a cleaved amplified polymorphic sequence (CAPS) marker that showed absolute identification accuracy (100%) of inflorescence type in a validation panel consisting of eighteen H. macrophylla cultivars. The SNP was investigated in 341 F1 progenies using genotyping by sequencing (GBS) and co-segregated with inflorescence type (χ 2 = 0.12; P = 0.73). The SNP was subsequently used for breeding selection using kompetitive allele specific PCR (KASP)...Continue Reading

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References

Nov 5, 1999·Methods in Molecular Biology·T G Burland
Nov 26, 2002·Trends in Genetics : TIG·Michael M NeffMichael Kalishman
Apr 6, 2005·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·I BertinM D Gale
Jun 17, 2008·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Anke LehmensiekRaymond B McNamara
Jan 22, 2010·Current Opinion in Plant Biology·J Antoni Rafalski
Oct 26, 2010·Nature Genetics·Xuehui HuangBin Han
May 17, 2011·PloS One·Robert J ElshireSharon E Mitchell
May 13, 2014·Methods in Molecular Biology·Chunlin HeJeffrey Anthony
Apr 9, 2015·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Meng LinGuihua Bai
Mar 24, 2016·Molecular Biology and Evolution·Sudhir KumarKoichiro Tamura
Nov 9, 2016·Nature Communications·Ke CaoLirong Wang
Dec 22, 2016·Frontiers in Plant Science·Dietmar F SchulzThomas Debener
Dec 6, 2017·Frontiers in Plant Science·Xingbo Wu, Matthew W Blair
May 2, 2018·Nature Genetics·Olivier RaymondMohammed Bendahmane
Jun 13, 2018·Nature Plants·L Hibrand Saint-OyantF Foucher

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Methods Mentioned

BETA
PCR
genotyping
electrophoresis

Software Mentioned

dCAPS Finder
TASSEL UNEAK
MEGA
STRUCTURE HARVESTER
GBS
Mophead
SAPHO
TASSEL
KASP
STRUCTURE

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