Genome-wide association study of rice (Oryza sativa L.) leaf traits with a high-throughput leaf scorer

Journal of Experimental Botany
Wanneng YangLizhong Xiong

Abstract

Leaves are the plant's solar panel and food factory, and leaf traits are always key issues to investigate in plant research. Traditional methods for leaf trait measurement are time-consuming. In this work, an engineering prototype has been established for high-throughput leaf scoring (HLS) of a large number of Oryza sativa accessions. The mean absolute per cent of errors in traditional measurements versus HLS were below 5% for leaf number, area, shape, and colour. Moreover, HLS can measure up to 30 leaves per minute. To demonstrate the usefulness of HLS in dissecting the genetic bases of leaf traits, a genome-wide association study (GWAS) was performed for 29 leaf traits related to leaf size, shape, and colour at three growth stages using HLS on a panel of 533 rice accessions. Nine associated loci contained known leaf-related genes, such as Nal1 for controlling the leaf width. In addition, a total of 73, 123, and 177 new loci were detected for traits associated with leaf size, colour, and shape, respectively. In summary, after evaluating the performance with a large number of rice accessions, the combination of GWAS and high-throughput leaf phenotyping (HLS) has proven a valuable strategy to identify the genetic loci controllin...Continue Reading

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Citations

Aug 25, 2016·Frontiers in Plant Science·Xiaojing DangDelin Hong
Dec 9, 2017·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Xinxin TangSibin Yu
Dec 15, 2017·Plant Methods·Konstantinos N BlazakisPanagiotis Kalaitzis
Sep 15, 2016·Development·Jyotirmaya MathanAashish Ranjan
Mar 23, 2019·Frontiers in Plant Science·Sheng WuYongjian Wang
Oct 30, 2015·Journal of Experimental Botany·Roland Pieruschka, Tracy Lawson
Jun 20, 2019·Frontiers in Plant Science·Chunjiang ZhaoJiangchuan Fan
Dec 4, 2020·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Huong Thi Mai ToNga T P Mai

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Methods Mentioned

BETA
genotyping
dissection

Software Mentioned

LabVIEW
GLAR
TASSEL
GEC

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