Genome-wide association study reveals novel players in defense hormone crosstalk in Arabidopsis.

Plant, Cell & Environment
Silvia ProiettiCorné M J Pieterse

Abstract

Jasmonic acid (JA) regulates plant defenses against necrotrophic pathogens and insect herbivores. Salicylic acid (SA) and abscisic acid (ABA) can antagonize JA-regulated defenses, thereby modulating pathogen or insect resistance. We performed a genome-wide association (GWA) study on natural genetic variation in Arabidopsis thaliana for the effect of SA and ABA on the JA pathway. We treated 349 Arabidopsis accessions with methyl JA (MeJA), or a combination of MeJA and either SA or ABA, after which expression of the JA-responsive marker gene PLANT DEFENSIN1.2 (PDF1.2) was quantified as a readout for GWA analysis. Both hormones antagonized MeJA-induced PDF1.2 in the majority of the accessions but with a large variation in magnitude. GWA mapping of the SA- and ABA-affected PDF1.2 expression data revealed loci associated with crosstalk. GLYI4 (encoding a glyoxalase) and ARR11 (encoding an Arabidopsis response regulator involved in cytokinin signalling) were confirmed by T-DNA insertion mutant analysis to affect SA-JA crosstalk and resistance against the necrotroph Botrytis cinerea. In addition, At1g16310 (encoding a cation efflux family protein) was confirmed to affect ABA-JA crosstalk and susceptibility to Mamestra brassicae herbiv...Continue Reading

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Citations

Mar 31, 2019·The Plant Journal : for Cell and Molecular Biology·Rishav RayIan T Baldwin
Nov 30, 2018·Plant, Cell & Environment·Anne CortlevenThomas Schmülling
Feb 23, 2020·Frontiers in Plant Science·Cinzia Margherita BerteaFrancesca Barbero
Oct 16, 2021·Nature Plants·Daan MertensErik H Poelman

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Methods Mentioned

BETA
genotyping
PCR
electrophoresis

Software Mentioned

LSM
FAST
R
LMM
AMM
GWAPP
SIGnAL
GWAPP Geneviewer

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