Genome-wide chromatin maps derived from limited numbers of hematopoietic progenitors.

Nature Methods
Mazhar AdliBradley E Bernstein

Abstract

Current methods for whole-genome mapping of protein-DNA interactions, performed by coupling chromatin immunoprecipitation with high-throughput sequencing (ChIP-seq), require large amounts of starting materials, which precludes their application to rare cell types. Here we combine a high-sensitivity ChIP assay with a new library preparation procedure to map histone modifications in as few as 10,000 cells. We used the technique to characterize mouse hematopoietic progenitors and thereby gain insight into their developmental program.

References

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Citations

Oct 26, 2013·Nature Communications·Christine S ChengIdo Amit
Sep 22, 2011·Nature Immunology·Benjamin L KidderKeji Zhao
Jun 7, 2011·Nature Methods·Pattabhiraman ShankaranarayananHinrich Gronemeyer
Sep 1, 2011·Nature Methods·Monya Baker
Oct 1, 2011·Nature Protocols·Mazhar Adli, Bradley E Bernstein
Jan 28, 2012·Nature Protocols·Pattabhiraman ShankaranarayananHinrich Gronemeyer
Dec 26, 2012·Proceedings of the National Academy of Sciences of the United States of America·Xinghua PanSherman M Weissman
Sep 18, 2013·Proceedings of the National Academy of Sciences of the United States of America·Bluma J LeschDavid C Page
Sep 18, 2010·Human Molecular Genetics·Ewan BirneyVishwanath R Iyer
Mar 3, 2012·Cold Spring Harbor Perspectives in Biology·Kornelia Polyak
Mar 2, 2011·BMC Genomics·Joshua W K HoPeter J Park
Nov 23, 2012·BMC Genomics·Gregor D GilfillanRobert Lyle
Jul 11, 2013·BMC Genomics·Sarah K BowmanRobert E Kingston
Jun 8, 2011·Epigenetics & Chromatin·Marco De GobbiDouglas R Higgs
Dec 30, 2011·Genome Biology·David J AdamsAlbertha J M Walhout
Dec 1, 2011·Epigenomics·Angela Bithell
Apr 12, 2012·Haematologica·Kirsten GrønbækUNKNOWN European Genomics and Epigenomics Study on MDS and AML (EuGESMA), COST Action BM0801
Mar 8, 2011·Yi chuan = Hereditas·Xing-Hua PanSadie L Marjani
Jan 7, 2015·Journal of Cellular Biochemistry·Mazhar AdliSamir S El-Dahr
Feb 2, 2016·Schizophrenia Research·John F FullardPanos Roussos
Dec 22, 2015·Molecular Cell·Peter van GalenBradley E Bernstein
Dec 26, 2015·Nucleic Acids Research·Laura ArrigoniUlrike Bönisch
Dec 23, 2015·BMC Genomics·Cristina ValensisiR David Hawkins
Jan 1, 2014·European Journal of Immunology·Maria Alessandra ViganoAntonius Rolink
Oct 13, 2015·Nature Biotechnology·Assaf RotemBradley E Bernstein
Oct 16, 2015·Nature Reviews. Genetics·Omer Schwartzman, Amos Tanay
Aug 29, 2012·Wiley Interdisciplinary Reviews. Systems Biology and Medicine·Gregory H Underhill
Jul 28, 2015·Nature Methods·Zhenning CaoChang Lu
Aug 19, 2015·Nature Methods·Christian SchmidlChristoph Bock
Jul 12, 2014·Experimental Cell Research·Jonathan I Sive, Berthold Göttgens
Aug 2, 2014·Cell·Bérénice A BenayounAnne Brunet

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