Genome-wide DNA methylation analysis in lung fibroblasts co-cultured with silica-exposed alveolar macrophages

Respiratory Research
Juan LiChang Fu Hao

Abstract

Exposure to crystalline silica is considered to increase the risk of lung fibrosis. The primary effector cell, the myofibroblast, plays an important role in the deposition of extracellular matrix (ECM). DNA methylation change is considered to have a potential effect on myofibroblast differentiation. Therefore, the present study was designed to investigate the genome-wide DNA methylation profiles of lung fibroblasts co-cultured with alveolar macrophages exposed to crystalline silica in vitro. AM/fibroblast co-culture system was established. CCK8 was used to assess the toxicity of AMs. mRNA and protein expression of collagen I, α-SMA, MAPK9 and TGF-β1 of fibroblasts after AMs exposed to 100 μg /ml SiO2 for 0-, 24-, or 48 h were determined by means of quantitative real-time PCR, immunoblotting and immunohistochemistry. Genomic DNA of fibroblasts was isolated using MeDIP-Seq to sequence. R software, GO, KEGG and Cytoscape were used to analyze the data. SiO2 exposure increased the expression of collagen I and α-SMA in fibroblasts in co-culture system. Analysis of fibroblast methylome identified extensive methylation changes involved in several signaling pathways, such as the MAPK signaling pathway and metabolic pathways. Several can...Continue Reading

References

Jul 1, 1993·The American Journal of the Medical Sciences·R A Clark
Jan 1, 1997·The International Journal of Biochemistry & Cell Biology·R P MarshallG J Laurent
Sep 5, 2001·Current Opinion in Genetics & Development·J M Slack, D Tosh
Nov 9, 2002·Bioinformatics·Long-Cheng Li, Rajvir Dahiya
Feb 9, 2005·Current Opinion in Pulmonary Medicine·Binaya RimalWilliam N Rom
Jan 19, 2006·Radiographics : a Review Publication of the Radiological Society of North America, Inc·Semin ChongTae Sung Kim
Jul 18, 2006·Nucleic Acids Research·Jia YeJun Wang
Mar 28, 2007·Journal of Cellular Physiology·Kenneth R CutroneoH Paul Ehrlich
Sep 19, 2008·Genome Biology·Yong ZhangX Shirley Liu
Nov 20, 2008·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Tetsuya MatsuguchiTomokazu Ohnishi
Jan 20, 2009·American Journal of Respiratory Cell and Molecular Biology·Nathan SandboNickolai O Dulin
Mar 6, 2009·Genome Biology·Ben LangmeadSteven L Salzberg
May 2, 2009·Cardiovascular Research·Sandra B HaudekMark L Entman
Jun 6, 2009·The American Journal of Pathology·William D HardieJames S Hagood
Jul 8, 2009·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Jan I HersethPer E Schwarze
Sep 29, 2009·Gastroenterology·Johannes KluweRobert F Schwabe
Jan 15, 2010·The Journal of Biological Chemistry·Matthew W C ChanChristopher A McCulloch
Apr 24, 2010·Nature Reviews. Molecular Cell Biology·Eric N Olson, Alfred Nordheim
Apr 11, 2012·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Maria Jesus PerugorriaJelena Mann
Aug 14, 2012·Gastroenterology·Yuping ChenAnna Mae Diehl
Sep 25, 2012·Nature Reviews. Drug Discovery·Rosemary J Akhurst, Akiko Hata
Aug 19, 2015·American Journal of Respiratory and Critical Care Medicine·Na XieGang Liu
Sep 1, 2015·The Journal of Biological Chemistry·Karen BernardVictor J Thannickal
Apr 26, 2016·International Journal of Molecular Sciences·Panagiotis DouvarasValentina Fossati
Apr 26, 2016·Laboratory Investigation; a Journal of Technical Methods and Pathology·Gang LiRi-Xing Zhou
May 12, 2016·Journal of Molecular and Cellular Cardiology·April Stempien-OteroJennifer Davis
Jun 18, 2016·Frontiers in Physiology·Zeribe C NwosuYan Liu

❮ Previous
Next ❯

Citations

Sep 15, 2020·Molecular Diagnosis & Therapy·Krystian BartczakWojciech J Piotrowski
Feb 17, 2021·Toxicology and Industrial Health·Zhao-Qiang ZhangRuining Xie

❮ Previous
Next ❯

Datasets Mentioned

BETA
GSE93116

Methods Mentioned

BETA
Immunoprecipitation
bronchoalveolar lavage
Protein Assay
electrophoresis
MeDIP-seq
PCR
methylation profiling
geranylgeranylation

Software Mentioned

Perl
Image
Cytoscape
SPSS17
bowtie
Model - based Analysis of MeDIP - Seq software ( MACS )
WebGestalt
Ensembl
Web Gestalt
Molecular Complex Detection

Related Concepts

Related Feeds

CFTR Mutant Structural Therapy

Over 1700 different mutations in the CFTR genes have been shown to cause cystic fibrosis. Here is the latest research on structural therapy for CFTR mutants.

Related Papers

Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases
Ying SunShu-Xun Sun
Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi
Xiaojing LiuHuaiqing Chen
© 2022 Meta ULC. All rights reserved