Genome-wide DNA methylation analysis of the regenerative and non-regenerative tissues in sika deer (Cervus nippon)

Gene
Chun YangHang Gao

Abstract

Deer antlers, the secondary organs of deer, are a unique model to study regeneration of organ/tissue in mammals. Pedicle periosteum (PP) is the key tissue type for antler regeneration. Based on our previous study, the DNA methylation was found to be the basic molecular mechanism underlying the antler regeneration. In this study, we compare the genome-wide DNA methylation level in regenerative tissues (the potentiated PP of antler, muscle, heart and liver) and non-regenerative tissue (the dormant PP) of deer by the fluorescence-labeled methylation-sensitive amplified polymorphism (F-MSAP) method. Our results showed that DNA methylation level was significantly lower in the regenerative tissues compared to the non-regenerative tissue (P < 0.05). Furthermore, 26 T-DMRs which displayed different methylated status in regenerative and non-regenerative tissues were identified by the MSAP method, and were further confirmed by Southern blot analysis. Taken together, our data suggest that DNA methylation, an important epigenetic regulation mechanism, may play an important role in the mammalian tissue/organ regeneration.

References

Aug 11, 2001·Science·P A Jones, D Takai
Jan 10, 2002·Genes & Development·Adrian Bird
May 4, 2004·European Journal of Morphology·C Li, J M Suttie
Jun 15, 2005·Annual Review of Biochemistry·Mary Grace Goll, Timothy H Bestor
Sep 13, 2005·Journal of Cellular Physiology·Daniela Palacios, Pier Lorenzo Puri
Oct 4, 2005·Molecular and Cellular Biology·Nibedita RathEdward E Morrisey
Jan 24, 2007·Gastroenterology·Domenico AlvaroGianfranco Alpini
Oct 16, 2007·Animal Biotechnology·Qing XuYing Yu
Jun 6, 2009·Current Stem Cell Research & Therapy·Chunyi LiAllan Sheppard
Dec 17, 2009·The New Phytologist·Koen J F VerhoevenArjen Biere
Aug 5, 2010·Nature Reviews. Molecular Cell Biology·Susan C Wu, Yi Zhang
Sep 28, 2010·Current Opinion in Plant Biology·Hidetoshi Saze, Tetsuji Kakutani
Feb 26, 2011·Science·Enzo R PorrelloHesham A Sadek
May 20, 2011·Nature·Michael A Laflamme, Charles E Murry
Sep 20, 2011·Cell·Nidhi BhutaniHelen M Blau
Feb 22, 2012·Trends in Cell Biology·Christof Niehrs, Andrea Schäfer
Mar 13, 2012·Annual Review of Biochemistry·Howard Cedar, Yehudit Bergman
Sep 15, 2012·Annual Review of Genetics·Don-Marc FranchiniSvend K Petersen-Mahrt
Feb 13, 2013·Nature Reviews. Genetics·Zachary D Smith, Alexander Meissner
Jul 25, 2013·Epigenetics : Official Journal of the DNA Methylation Society·Kentaro HiroseYutaka Kikuchi
Aug 10, 2013·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Bartosz GórnikiewiczPaweł Sachadyn
Jan 29, 2014·Preventive Nutrition and Food Science·Kyung-Mi ChangGun-Hee Kim
Feb 6, 2014·Translational Research : the Journal of Laboratory and Clinical Medicine·Shennen A MaoScott L Nyberg
Feb 15, 2014·Developmental Cell·Margaret Buckingham, Peter W J Rigby
May 28, 2014·Cellular Signalling·Jeffrey J HeardFuyuhiko Tamanoi
Jun 21, 2014·Trends in Biochemical Sciences·Albert Jeltsch, Renata Z Jurkowska
Nov 18, 2014·The Journal of Clinical Investigation·Claus KordesDieter Häussinger
Dec 11, 2014·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Choon Boon SimEnzo R Porrello
Jun 13, 2015·PloS One·Silke GötzeDieter Häussinger
Dec 29, 2015·Frontiers in Bioscience (Landmark Edition)·Chunyi Li, Wenhui Chu
Mar 22, 2016·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Kosuke KajiSnorri S Thorgeirsson
Oct 30, 2016·International Journal of Molecular Sciences·Zhen DongChunyi Li

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