Genome-wide DNA methylation analysis on C-reactive protein among Ghanaians suggests molecular links to the emerging risk of cardiovascular diseases.

NPJ Genomic Medicine
Felix P ChilungaCharles Agyemang

Abstract

Molecular mechanisms at the intersection of inflammation and cardiovascular diseases (CVD) among Africans are still unknown. We performed an epigenome-wide association study to identify loci associated with serum C-reactive protein (marker of inflammation) among Ghanaians and further assessed whether differentially methylated positions (DMPs) were linked to CVD in previous reports, or to estimated CVD risk in the same population. We used the Illumina Infinium® HumanMethylation450 BeadChip to obtain DNAm profiles of blood samples in 589 Ghanaians from the RODAM study (without acute infections, not taking anti-inflammatory medications, CRP levels < 40 mg/L). We then used linear models to identify DMPs associated with CRP concentrations. Post-hoc, we evaluated associations of identified DMPs with elevated CVD risk estimated via ASCVD risk score. We also performed subset analyses at CRP levels ≤10 mg/L and replication analyses on candidate probes. Finally, we assessed for biological relevance of our findings in public databases. We subsequently identified 14 novel DMPs associated with CRP. In post-hoc evaluations, we found that DMPs in PC, BTG4 and PADI1 showed trends of associations with estimated CVD risk, we identified a separat...Continue Reading

References

May 7, 1999·The Biochemical Journal·S Jitrapakdee, J C Wallace
Feb 14, 2006·The American Journal of Clinical Nutrition·Peter Libby
Dec 7, 2007·The American Journal of Medicine·Ravi DhingraChristopher J O'Donnell
Jun 6, 2009·Journal of Dental Research·S P Barros, S Offenbacher
Aug 7, 2010·Database : the Journal of Biological Databases and Curation·Marilyn SafranDoron Lancet
Jan 15, 2011·Nature Reviews. Immunology·Marc Y Donath, Steven E Shoelson
Feb 16, 2011·Nature Immunology·Göran K Hansson, Andreas Hermansson
Jul 12, 2011·Epigenetics : Official Journal of the DNA Methylation Society·Fang Fang ZhangAlfredo Morabia
May 10, 2012·BMC Bioinformatics·Eugene Andres HousemanKarl T Kelsey
Jul 23, 2013·BMC Medical Genetics·Mary K WojczynskiMuredach P Reilly
Nov 30, 2013·Biochimica Et Biophysica Acta·Beatriz Sánchez-SolanaRakesh Kumar
Mar 19, 2014·Lancet·Katherine J DickNilesh J Samani
Jan 13, 2015·American Journal of Human Genetics·Hansjörg BaurechtSara J Brown
Aug 11, 2015·Open Forum Infectious Diseases·Anurag Markanday
Dec 3, 2016·Nucleic Acids Research·Jacqueline MacArthurHelen Parkinson
Dec 17, 2016·Epigenetics & Chromatin·Shan V AndrewsM Daniele Fallin
Dec 19, 2016·American Journal of Human Genetics·Ari V Ahola-OlliOlli T Raitakari
Jun 24, 2017·Nutrients·Frédéric GuénardMarie-Claude Vohl
Nov 19, 2017·Circulation. Cardiovascular Quality and Outcomes·Daniel BoatengKerstin Klipstein-Grobusch
Jan 25, 2018·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Udom Lao-OnSarawut Jitrapakdee
Apr 6, 2018·Human Genome Variation·Shohei KomakiAtsushi Shimizu
Jan 1, 2019·American Journal of Human Genetics·Gleb KichaevAlkes L Price
Aug 21, 2019·Brain : a Journal of Neurology·Jaeyoon ChungChristopher D Anderson
Nov 2, 2019·The Journal of Allergy and Clinical Immunology·Zhaozhong ZhuLiming Liang

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Software Mentioned

MissMethyl
OpenSea
MethylAid
bumphunter
Bioconductor
RODAM
IMETHYL
minfi
R

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