Genome-wide DNA methylation profiles indicate CD8+ T cell hypermethylation in multiple sclerosis

PloS One
Steffan D BosLisa F Barcellos

Abstract

Determine whether MS-specific DNA methylation profiles can be identified in whole blood or purified immune cells from untreated MS patients. Whole blood, CD4+ and CD8+ T cell DNA from 16 female, treatment naïve MS patients and 14 matched controls was profiled using the HumanMethylation450K BeadChip. Genotype data were used to assess genetic homogeneity of our sample and to exclude potential SNP-induced DNA methylation measurement errors. As expected, significant differences between CD4+ T cells, CD8+ T cells and whole blood DNA methylation profiles were observed, regardless of disease status. Strong evidence for hypermethylation of CD8+ T cell, but not CD4+ T cell or whole blood DNA in MS patients compared to controls was observed. Genome-wide significant individual CpG-site DNA methylation differences were not identified. Furthermore, significant differences in gene DNA methylation of 148 established MS-associated risk genes were not observed. While genome-wide significant DNA methylation differences were not detected for individual CpG-sites, strong evidence for DNA hypermethylation of CD8+ T cells for MS patients was observed, indicating a role for DNA methylation in MS. Further, our results suggest that large DNA methylatio...Continue Reading

References

Oct 8, 1998·Multiple Sclerosis : Clinical and Laboratory Research·P StinissenJ Raus
Dec 20, 2003·Nature·UNKNOWN International HapMap Consortium
May 22, 2004·Nature Reviews. Genetics·UNKNOWN International HapMap Consortium
May 25, 2007·Nature·Andrew P Feinberg
May 29, 2007·International Review of Neurobiology·Tanuja Chitnis
Jul 31, 2007·The New England Journal of Medicine·UNKNOWN International Multiple Sclerosis Genetics ConsortiumStephen L Hauser
Oct 15, 2008·Annual Review of Pharmacology and Toxicology·Moshe Szyf
Oct 31, 2008·Lancet·Alastair Compston, Alasdair Coles
Mar 10, 2011·Annals of Neurology·Chris H PolmanJerry S Wolinsky
Aug 16, 2011·Genomics·Marina BibikovaRichard Shen
Sep 1, 2011·Multiple Sclerosis : Clinical and Laboratory Research·Roberta CalabresePaola Caiafa
Dec 1, 2011·Epigenomics·Sarah DedeurwaerderFrançois Fuks
Feb 10, 2012·Journal of the American Medical Informatics Association : JAMIA·Carrie C BuchananMarylyn D Ritchie
Mar 17, 2012·International Journal of Epidemiology·Andrew E JaffeRafael A Irizarry
May 9, 2012·Frontiers in Immunology·Eric S HusebyBrian D Stadinski
Jun 26, 2012·Immunological Reviews·Pierre-Antoine GourraudSergio E Baranzini
Sep 8, 2012·Science·Matthew T MauranoJohn A Stamatoyannopoulos
Nov 7, 2012·Nature·UNKNOWN 1000 Genomes Project ConsortiumGil A McVean
Nov 15, 2012·Sub-cellular Biochemistry·Helene Myrtue Nielsen, Jörg Tost
May 2, 2013·Genome Medicine·John W WhitakerGary S Firestein
May 22, 2013·PloS One·Sonja ZeilingerThomas Illig
Jun 5, 2013·American Journal of Human Genetics·UNKNOWN International Multiple Sclerosis Genetics Consortium
Aug 27, 2013·Critical Reviews in Immunology·Bieke BrouxNiels Hellings
Oct 1, 2013·Nature Genetics·UNKNOWN International Multiple Sclerosis Genetics Consortium (IMSGC)Jacob L McCauley
Nov 26, 2013·Nature Neuroscience·Jimmy L HuynhPatrizia Casaccia
Dec 18, 2013·Multiple Sclerosis : Clinical and Laboratory Research·M C GravesJ Lechner-Scott
Jan 24, 2014·Bioinformatics·Eugene Andres HousemanCarmen J Marsit

❮ Previous
Next ❯

Citations

Jan 29, 2016·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Bao SunWei Zhang
Dec 30, 2015·Journal of Neuroimmunology·Paolo FagoneFerdinando Nicoletti
Mar 8, 2016·European Journal of Neurology : the Official Journal of the European Federation of Neurological Societies·S D BosH F Harbo
Jun 19, 2016·BMC Genetics·K Y NevenV Bollati
Sep 9, 2016·Auto- Immunity Highlights·Maria SokratousGeorgios M Hadjigeorgiou
Dec 10, 2016·Nature Reviews. Neurology·Tomas OlssonLars Alfredsson
Mar 9, 2018·Journal of Molecular Neuroscience : MN·Maria SokratousGeorgios M Hadjigeorgiou
Apr 26, 2018·Multiple Sclerosis : Clinical and Laboratory Research·Yiyi Ma, Philip L De Jager
Jul 30, 2017·Physiological Genomics·Galina Y ZheleznyakovaLara Kular
Jan 9, 2018·Multiple Sclerosis : Clinical and Laboratory Research·Kamilah Castro, Patrizia Casaccia
Jul 8, 2016·Science Advances·Till F M AndlauerBertram Müller-Myhsok
Oct 15, 2019·Immunological Reviews·Claire LoiseauDenise L Doolan
Nov 28, 2018·Scientific Reports·Vicki E MaltbyJeannette Lechner-Scott
Jul 13, 2016·Journal of Cellular Physiology·Li DuanDaping Wang
Aug 23, 2017·Expert Opinion on Therapeutic Targets·Heng LiJianping Zuo
Mar 24, 2018·Brain and Behavior·Vanesa PytelJorge Matías-Guiu
Jul 25, 2018·Multiple Sclerosis Journal - Experimental, Translational and Clinical·Vicki E MaltbyJeannette Lechner-Scott
May 31, 2018·The Journal of Clinical Endocrinology and Metabolism·Sonia García-CalzónCharlotte Ling
May 7, 2019·Frontiers in Immunology·Qiancheng DengRong Xiao
Jul 15, 2017·Molecular Neurobiology·Marina DunaevaGer J M Pruijn
Jan 16, 2020·Journal of Neuroinflammation·Naiara Celarain, Jordi Tomas-Roig
Feb 23, 2020·The International Journal of Neuroscience·Khosro JamebozorgiAmirhossein Sahebkar
Jul 8, 2016·Neuromolecular Medicine·Saeed AslaniMahmoud Jafarnejad
May 9, 2019·Nature Communications·Nicole Y SourenJörn Walter
Dec 5, 2019·Frontiers in Genetics·Naiara Celarain, Jordi Tomas-Roig
Nov 4, 2016·Nature Reviews. Drug Discovery·David F ToughRab K Prinjha
Feb 13, 2021·International Journal of Molecular Sciences·Natalia BaulinaOlga Favorova
Dec 19, 2020·Scientific Reports·Vicki E MaltbyJeannette Lechner-Scott
May 16, 2021·Journal of Neuroimmunology·J M Garcia-ManteigaF Martinelli-Boneschi
Jun 11, 2021·Frontiers in Aging Neuroscience·María Olimpia Paz AlvarengaRafael Rodrigues Lima
Sep 22, 2021·Antioxidants & Redox Signaling·Xiaoqing Zheng, Amr H Sawalha
Dec 17, 2021·Proceedings of the National Academy of Sciences of the United States of America·Qin MaJorge R Oksenberg

❮ Previous
Next ❯

Methods Mentioned

BETA
methylation profiling
PCA

Software Mentioned

BeadStudio
R
IMPUTE2

Related Concepts

Related Feeds

Adenomatous Polyposis Coli

Adenomatous polyposis coli is a protein encoded by the APC gene and acts as a tumor suppressor. Discover the latest research on adenomatous polyposis coli here.

Autoimmune Diseases

Autoimmune diseases occur as a result of an attack by the immune system on the body’s own tissues resulting in damage and dysfunction. There are different types of autoimmune diseases, in which there is a complex and unknown interaction between genetics and the environment. Discover the latest research on autoimmune diseases here.

Cancer Genomics (Keystone)

Cancer genomics approaches employ high-throughput technologies to identify the complete catalog of somatic alterations that characterize the genome, transcriptome and epigenome of cohorts of tumor samples. Discover the latest research using such technologies in this feed.