Genome-wide DNA methylation profiles of porcine ovaries in estrus and proestrus

Physiological Genomics
Xiaolong ZhouAyong Zhao

Abstract

DNA methylation is an important epigenetic modification involved in the estrous cycle and the regulation of reproduction. Here, we investigated the genome-wide profiles of DNA methylation in porcine ovaries in proestrus and estrus using methylated DNA immunoprecipitation sequencing. The results showed that DNA methylation was enriched in intergenic and intron regions. The methylation levels of coding regions were higher than those of the 5'- and 3'-flanking regions of genes. There were 4,813 differentially methylated regions (DMRs) of CpG islands in the estrus vs. proestrus ovarian genomes. Additionally, 3,651 differentially methylated genes (DMGs) were identified in pigs in estrus and proestrus. The DMGs were significantly enriched in biological processes and pathways related to reproduction and hormone regulation. We identified 90 DMGs associated with regulating reproduction in pigs. Our findings can serve as resources for DNA methylome research focused on porcine ovaries and further our understanding of epigenetically regulated reproduction in mammals.

References

Apr 27, 2000·Endocrine Reviews·E A McGee, A J Hsueh
Jun 24, 2000·Reviews of Reproduction·M G Hunter
Jan 11, 2002·Developmental Biology·T R HainesP J Ainsworth
Apr 16, 2003·Cell Cycle·Peter A Jones
Oct 19, 2004·Seminars in Cancer Biology·Andrew P Feinberg
Jan 14, 2005·Journal of Cellular Physiology·Maria Irene ScaranoMaurizio D'Esposito
Jan 20, 2005·Journal of the National Cancer Institute·Long-Cheng LiRajvir Dahiya
Jul 7, 2005·Domestic Animal Endocrinology·A MadejS Einarsson
Jul 27, 2005·Hormones and Behavior·Alexander S KauffmanEmilie F Rissman
Sep 2, 2005·Nature Reviews. Genetics·Keith D Robertson
Dec 14, 2005·Animal Biotechnology·Lawrence SchookDouglas Smith
Jan 25, 2006·Proceedings of the National Academy of Sciences of the United States of America·Serge SaxonovDouglas L Brutlag
Feb 27, 2007·Cell·Aaron D GoldbergEmily Bernstein
Jun 7, 2007·Journal of Animal Breeding and Genetics = Zeitschrift Für Tierzüchtung Und Züchtungsbiologie·J F YuanS J Zhang
Jun 22, 2007·General and Comparative Endocrinology·Wu Dong, Kristine L Willett
Nov 13, 2008·Journal of Mammary Gland Biology and Neoplasia·Dedra H Fagan, Douglas Yee
Jan 16, 2009·Reproduction in Domestic Animals = Zuchthygiene·K GieseckeO Distl
May 9, 2009·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·José M GuzmánEvaristo L Mañanós
Nov 27, 2009·Fertility and Sterility·Ning XuMark O Goodarzi
Feb 6, 2010·Genome Research·Louise LaurentChia-Lin Wei
Jul 27, 2010·Epigenomics·Shaun D FouseJoseph F Costello
Mar 15, 2011·Animal Reproduction Science·N M SoedeB Kemp
Apr 15, 2011·Nature·Kristine WilliamsKristian Helin
May 17, 2011·PloS One·Qinghe LiNing Li
Aug 9, 2011·Stem Cells·Heather J LeeChristopher J Ormandy
Oct 4, 2011·Nature·Sanjeev ShuklaShalini Oberdoerffer
Mar 10, 2012·Nature Protocols·Oluwatosin TaiwoLee M Butcher
May 24, 2012·Nature Communications·Mingzhou LiRuiqiang Li
May 30, 2012·Nature Reviews. Genetics·Peter A Jones
Nov 16, 2012·Nature·Martien A M GroenenLawrence B Schook
Jan 29, 2013·Nature Neuroscience·Alejandro LomnicziSergio R Ojeda
May 9, 2013·International Journal of Endocrinology·Justyna BarćEwa Łucja Gregoraszczuk

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Methods Mentioned

BETA
immunoprecipitation
MBD-Seq
WGBS
RRBS
MeDIP-Seq
methyl-seq
chip
PCR
methylation

Software Mentioned

R Bioconductor
NGS QC Toolkit
SPSS
MedIP
MEDIPS
Seq
qPCR
edger Bio - conductor
KOBAS

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