Nov 14, 2015

Genome-Wide Estimates of Mutation Rates and Spectrum in Schizosaccharomyces pombe Indicate CpG Sites are Highly Mutagenic Despite the Absence of DNA Methylation

G3 : Genes - Genomes - Genetics
Megan G Behringer, David W Hall

Abstract

We accumulated mutations for 1952 generations in 79 initially identical, haploid lines of the fission yeast Schizosaccharomyces pombe, and then performed whole-genome sequencing to determine the mutation rates and spectrum. We captured 696 spontaneous mutations across the 79 mutation accumulation (MA) lines. We compared the mutation spectrum and rate to a recently published equivalent experiment on the same species, and to another model ascomycetous yeast, the budding yeast Saccharomyces cerevisiae. While the two species are approximately 600 million years diverged from each other, they share similar life histories, genome size and genomic G/C content. We found that Sc. pombe and S. cerevisiae have similar mutation rates, but Sc. pombe exhibits a stronger insertion bias. Intriguingly, we observed an increased mutation rate at cytosine nucleotides, specifically CpG nucleotides, which is also seen in S. cerevisiae. However, the absence of methylation in Sc. pombe and the pattern of mutation at these sites, primarily C → A as opposed to C → T, strongly suggest that the increased mutation rate is not caused by deamination of methylated cytosines. This result implies that the high mutability of CpG dinucleotides in other species may...Continue Reading

  • References65
  • Citations23

References

  • References65
  • Citations23

Citations

Mentioned in this Paper

Genome-Wide Association Study
Study
DNA Methylation [PE]
Size
Patterns
Noonan SYNDROME-LIKE Disorder With OR Without Juvenile Myelomonocytic Leukemia
Protein Methylation
Genome
Saccharomyces cerevisiae allergenic extract
cytidylyl-3'-5'-guanosine

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