Aug 27, 2015

Genome wide estimates of mutation rates and spectrum in Schizosaccharomyces pombe indicate CpG sites are highly mutagenic despite the absence of DNA methylation

BioRxiv : the Preprint Server for Biology
Megan G Behringer, David W Hall

Abstract

We accumulated mutations for 1952 generations in 79 initially identical, haploid lines of the fission yeast Schizosaccharomyces pombe and then performed whole-genome sequencing to determine the mutation rates and spectrum. We captured 696 spontaneous mutations across the 79 mutation accumulation lines. We compared the mutation spectrum and rate to another model ascomycetous yeast, the budding yeast Saccharomyces cerevisiae. While the two organisms are approximately 600 million years diverged from each other, they share similar life histories, genome size and genomic G/C content. We found that Sc. pombe and S. cerevisiae have similar mutation rates, contrary to what was expected given Sc. pombe’s smaller reported effective population size. Sc. pombe’s also exhibits a strong insertion bias in comparison to S. cerevisiae,. Intriguingly, we observed an increased mutation rate at cytosine nucleotides, specifically CpG nucleotides, which is also seen in S. cerevisiae. However, the absence of methylation in Sc. pombe and the pattern of mutation at these sites, primarily C→ A as opposed to C→T, strongly suggest that the increased mutation rate is not caused by deamination of methylated cytosines. This result implies that the high mutab...Continue Reading

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Mentioned in this Paper

DNA Methylation [PE]
Size
Patterns
Protein Methylation
Genome
Saccharomyces cerevisiae allergenic extract
cytidylyl-3'-5'-guanosine
Genomic Stability
Cytosine
Methylate

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