Genome-wide expression profiling Drosophila melanogaster deficiency heterozygotes reveals diverse genomic responses.

BioRxiv : the Preprint Server for Biology
Hangnoh LeeBrian Oliver


Deletions, commonly referred to as deficiencies by Drosophila geneticists, are valuable tools for mapping genes and for genetic pathway discovery via dose-dependent suppressor and enhancer screens. More recently, it has become clear that deviations from normal gene dosage are associated with multiple disorders in a range of species including humans. While we are beginning to understand some of the transcriptional effects brought about by gene dosage changes and the chromosome rearrangement breakpoints associated with them, much of this work relies on isolated examples. We have systematically examined deficiencies on the left arm of chromosome 2 and characterize gene-by-gene dosage responses that vary from collapsed expression through modest partial dosage compensation to full or even over compensation. We found negligible long-range effects of creating novel chromosome domains at deletion breakpoints, suggesting that cases of changes in gene regulation due to altered nuclear architecture are rare. These rare cases include trans de-repression when deficiencies delete chromatin characterized as repressive in other studies. Generally, effects of breakpoints on expression are promoter proximal (~100 bp) or within the gene body. Gen...Continue Reading

Related Concepts

Genome-Wide Association Study
Biochemical Pathway
Body Structure
Transcription, Genetic
Genomic Stability
Gene Deletion Abnormality
Gene Dosage Compensation Mechanism

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