Genome wide expression profiling of p53 regulated miRNAs in neuroblastoma

Scientific Reports
Ali RihaniTom Van Maerken

Abstract

Restoration of the antitumor activity of p53 could offer a promising approach for the treatment of neuroblastoma. MicroRNAs (miRNAs) are important mediators of p53 activity, but their role in the p53 response has not yet been comprehensively addressed in neuroblastoma. Therefore, we set out to characterize alterations in miRNA expression that are induced by p53 activation in neuroblastoma cells. Genome-wide miRNA expression analysis showed that miR-34a-5p, miR-182-5p, miR-203a, miR-222-3p, and miR-432-5p are upregulated following nutlin-3 treatment in a p53 dependent manner. The function of miR-182-5p, miR-203a, miR-222-3p, and miR-432-5p was analyzed by ectopic overexpression of miRNA mimics. We observed that these p53-regulated miRNAs inhibit the proliferation of neuroblastoma cells to varying degrees, with the most profound growth inhibition recorded for miR-182-5p. Overexpression of miR-182-5p promoted apoptosis in some neuroblastoma cell lines and induced neuronal differentiation of NGP cells. Using Chromatin Immunoprecipitation-qPCR (ChIP-qPCR), we did not observe direct binding of p53 to MIR182, MIR203, MIR222, and MIR432 in neuroblastoma cells. Taken together, our findings yield new insights in the network of p53-regula...Continue Reading

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Citations

Feb 2, 2017·Journal of Cellular Physiology·Sharareh GholaminAmir Avan
Feb 24, 2017·Journal of Biosciences·Daiane Teixeira DE OliveiraGlenda Nicioli DA Silva
Feb 28, 2016·British Journal of Pharmacology·P Urbánek, L-O Klotz
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Jul 25, 2018·Molecular Cancer Research : MCR·Priyanka KulkarniRajvir Dahiya
Jan 29, 2021·International Journal of Molecular Sciences·Nikhil R GandasiSonchita Bagchi
Mar 16, 2021·Frontiers in Oncology·Omidvar RezaeiSoudeh Ghafouri-Fard
Nov 22, 2016·The American Journal of Pathology·Julia WilflingsederRainer Oberbauer

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Datasets Mentioned

BETA
GSM783262
GSM501692

Methods Mentioned

BETA
xenografts
transfection
ChIP-Seq
scraping
PCR
FCS
Protein Assay
Immunoprecipitation
Chip

Software Mentioned

Isis FISH Imaging System
Experion
Primer
SPSS
SDS
stats
Bowtie
R
Integrative Genomics Viewer ( IGV )
qbase + qPCR analysis

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