Genome-Wide Gene Expression Profiles Analysis Reveal Novel Insights into Drought Stress in Foxtail Millet (Setaria italica L.)

International Journal of Molecular Sciences
Ling QinYing Ruan

Abstract

Foxtail millet (Setaria italica (L.) P. Beauv) is an important food and forage crop because of its health benefits and adaptation to drought stress; however, reports of transcriptomic analysis of genes responding to re-watering after drought stress in foxtail millet are rare. The present study evaluated physiological parameters, such as proline content, p5cs enzyme activity, anti-oxidation enzyme activities, and investigated gene expression patterns using RNA sequencing of the drought-tolerant foxtail millet variety (Jigu 16) treated with drought stress and rehydration. The results indicated that drought stress-responsive genes were related to many multiple metabolic processes, such as photosynthesis, signal transduction, phenylpropanoid biosynthesis, starch and sucrose metabolism, and osmotic adjustment. Furthermore, the Δ1-pyrroline-5-carboxylate synthetase genes, SiP5CS1 and SiP5CS2, were remarkably upregulated in foxtail millet under drought stress conditions. Foxtail millet can also recover well on rehydration after drought stress through gene regulation. Our data demonstrate that recovery on rehydration primarily involves proline metabolism, sugar metabolism, hormone signal transduction, water transport, and detoxificatio...Continue Reading

References

Jan 1, 1984·Methods in Enzymology·H Aebi
Dec 28, 1999·Proceedings of the National Academy of Sciences of the United States of America·X Qin, J A Zeevaart
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Sep 18, 2002·Trends in Plant Science·Ron Mittler
Sep 16, 2003·Current Opinion in Plant Biology·Kazuo ShinozakiMotoaki Seki
Feb 1, 1980·Plant Physiology·R R Stewart, J D Bewley
May 4, 2006·Annual Review of Plant Biology·Ajit NottJoanne Chory
Nov 1, 2006·Journal of Experimental Botany·Kazuo Shinozaki, Kazuko Yamaguchi-Shinozaki
Nov 1, 2007·The Plant Journal : for Cell and Molecular Biology·Gyöngyi SzékelyLászló Szabados
Feb 7, 2008·Annals of Botany·Peter M Neumann
Apr 2, 2008·Amino Acids·Nathalie Verbruggen, Christian Hermans
Jun 3, 2008·Nature Methods·Ali MortazaviBarbara Wold
Nov 17, 2009·Biotechnology Advances·M Ashraf
Dec 29, 2009·Trends in Plant Science·László Szabados, Arnould Savouré
Apr 13, 2010·Trends in Plant Science·Jose L Pruneda-Paz, Steve A Kay
Mar 19, 2011·Plant Signaling & Behavior·Marija SpoljarevićTihana Teklić
Apr 5, 2011·Journal of Experimental Botany·Pinghua Li, Thomas P Brutnell
May 15, 2012·Nature Biotechnology·Jeffrey L BennetzenKatrien M Devos
Oct 16, 2012·BMC Plant Biology·Dietmar FunckGiuseppe Forlani
Jun 12, 2014·Functional & Integrative Genomics·Mehanathan MuthamilarasanManoj Prasad
Feb 19, 2015·Nature Biotechnology·Mihaela PerteaSteven L Salzberg
Dec 27, 2016·Frontiers in Plant Science·Shankar R PantKranthi K Mandadi
Aug 7, 2018·Plant Science : an International Journal of Experimental Plant Biology·Imen KlayJulien Pirrello
Nov 15, 2018·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Dorsaf HmidiDorsaf Messedi
Feb 14, 2019·Functional & Integrative Genomics·Roshan Kumar SinghManoj Prasad
Feb 20, 2019·International Journal of Molecular Sciences·Xiaohui YangLiping Jin

❮ Previous
Next ❯

Methods Mentioned

BETA
RNA-Seq
PCR
MDA
ELISA
Assay
confocal microscopy

Software Mentioned

StringTie
clusterProfiler R package
DESeq 2 R
HISAT2
SPSS

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.