Genome-wide hypomethylation of LINE-1 and Alu retroelements in cell-free DNA of blood is an epigenetic biomarker of human aging

Saudi Journal of Biological Sciences
Lars ErichsenSimeon Santourlidis

Abstract

Aging associated DNA hypomethylation of LINE-1 and Alu retroelements may be a crucial determinant of loss of genomic integrity, deterioration and cancer. In peripheral blood LINE-1 hypomethylation has been reported to increase during aging, but other studies did not observe significant changes. We hypothesized that these apparently inconsistent reports might relate to differences between cellular and cell-free DNA. Using the technique of idiolocal normalization of real-time methylation-specific PCR (IDLN-MSP) for genetic imbalanced DNA specimens we obtained evidence that LINE-1 hypomethylation in cell-free DNA, but not cellular DNA from peripheral blood is an epigenetic biomarker for human aging. Furthermore, hypomethylation of cell-free DNA is more extensive in smokers, suggesting that it might be used as a surrogate marker for monitoring the improvement of smoking-induced adverse effects after cancelling smoking.

Citations

Feb 23, 2019·Frontiers in Genetics·Ivana Babić BožovićBojana Brajenović-Milić
Sep 6, 2020·Cells·Anastasia A PonomaryovaTatyana L Azhikina
Jul 18, 2019·International Journal of Molecular Sciences·Adolfo A PazBernardo J Krause
Oct 20, 2020·Cellular and Molecular Neurobiology·Nuraqila Mohd MurshidSuzana Makpol
Jan 15, 2021·Thorax·Ana I Hernandez CorderoUNKNOWN INSIGHT START Pulmonary and Genomic Substudy Groups
Jun 5, 2021·Epigenetics & Chromatin·Xena Giada Pappalardo, Viviana Barra
Aug 22, 2021·Experimental Eye Research·Jing WuJia-Ning Zhong

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BETA
M80343.1

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BETA
PCR

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StepOne

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