Genome-wide identification and characterization of cacao WRKY transcription factors and analysis of their expression in response to witches' broom disease

PloS One
Dayanne Silva Monteiro de AlmeidaFabienne Micheli

Abstract

Transcriptional regulation, led by transcription factors (TFs) such as those of the WRKY family, is a mechanism used by the organism to enhance or repress gene expression in response to stimuli. Here, we report on the genome-wide analysis of the Theobroma cacao WRKY TF family and also investigate the expression of WRKY genes in cacao infected by the fungus Moniliophthora perniciosa. In the cacao genome, 61 non-redundant WRKY sequences were found and classified in three groups (I to III) according to the WRKY and zinc-finger motif types. The 61 putative WRKY sequences were distributed on the 10 cacao chromosomes and 24 of them came from duplication events. The sequences were phylogenetically organized according to the general WRKY groups. The phylogenetic analysis revealed that subgroups IIa and IIb are sister groups and share a common ancestor, as well as subgroups IId and IIe. The most divergent groups according to the plant origin were IIc and III. According to the phylogenetic analysis, 7 TcWRKY genes were selected and analyzed by RT-qPCR in susceptible and resistant cacao plants infected (or not) with M. perniciosa. Some TcWRKY genes presented interesting responses to M. perniciosa such as Tc01_p014750/Tc06_p013130/AtWRKY28...Continue Reading

References

Apr 1, 1998·Bioinformatics·T L Bailey, M Gribskov
Jan 27, 1999·Bioinformatics·D Posada, K A Crandall
Apr 6, 1999·The Journal of Hand Surgery : Journal of the British Society for Surgery of the Hand·E OrhunU Kantarci
Apr 29, 2000·Trends in Plant Science·T EulgemI E Somssich
Apr 18, 2002·Current Opinion in Structural Biology·Stephen K Burley, Katsuhiko Kamada
Jan 1, 1996·Annual Review of Phytopathology·L H Purdy, R A Schmidt
Mar 23, 2004·Nucleic Acids Research·Robert C Edgar
May 19, 2004·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·James W BorroneRaymond J Schnell
Jan 5, 2005·BMC Evolutionary Biology·Yuanji Zhang, Liangjiang Wang
Oct 26, 2005·Journal of Computational Biology : a Journal of Computational Molecular Cell Biology·Sheng Zhao, Russell D Fernald
Jun 15, 2007·Annals of Botany·Abelmon S GesteiraJúlio C M Cascardo
Aug 8, 2007·Molecular Phylogenetics and Evolution·James W BorroneRaymond J Schnell
Mar 11, 2008·Cell Host & Microbe·Jeffrey CaplanSavithramma P Dinesh-Kumar
Apr 9, 2008·Journal of Experimental Botany·Gerben van OoijenFrank L W Takken
Aug 16, 2008·The Plant Journal : for Cell and Molecular Biology·M Shahid MukhtarImre E Somssich
May 22, 2009·Nucleic Acids Research·Timothy L BaileyWilliam S Noble
Mar 23, 2010·Trends in Plant Science·Paul J RushtonQingxi J Shen
Apr 7, 2010·Molecular Plant-microbe Interactions : MPMI·Ligang ChenDiqiu Yu
Dec 15, 2010·The Plant Journal : for Cell and Molecular Biology·Shree P PandeyImre E Somssich
Dec 28, 2010·Nature Genetics·Xavier ArgoutClaire Lanaud
Jun 7, 2011·Plant Physiology and Biochemistry : PPB·Cristiano Villela DiasJúlio Cézar de Mattos Cascardo
Aug 2, 2011·Genetica·Qishan WangYuchun Pan
Nov 19, 2011·Genetics and Molecular Research : GMR·T T PinheiroA Figueira
Jan 6, 2012·Nucleic Acids Research·Yupeng WangAndrew H Paterson
Jan 24, 2012·Journal of Experimental Botany·Sébastien BesseauE Tapio Palva
May 10, 2012·Molecular Genetics and Genomics : MGG·Shengxiong HuangYongsheng Liu
Oct 17, 2012·The Plant Journal : for Cell and Molecular Biology·Jae Sung ShimYang Do Choi
May 30, 2013·BMJ Case Reports·Prabha Nini GuptaPraveen Vellappan
Jun 3, 2014·BioMed Research International·Ying Zhang, Jian Can Feng

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Methods Mentioned

BETA
electrophoresis
PCR
transgenic

Software Mentioned

CocoaGenDB
MxPro QPCR
SASM
LATEST
MEME
Agri
ClustalOMEGA
MCScanX
Modeltest
[UNK] Designer

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