Genome-wide identification and evaluation of novel internal control genes for Q-PCR based transcript normalization in wheat.

Plant Molecular Biology
Xiang-Yu LongYou-Liang Zheng

Abstract

To accurately quantify gene expression using quantitative PCR amplification, it is vital that one or more ideal internal control genes are used to normalize the samples to be compared. Ideally, the expression level of those internal control genes should vary as little as possible between tissues, developmental stages and environmental conditions. In this study, 32 candidate genes for internal control were obtained from the analysis of nine independent experiments which included 333 Affymetrix GeneChip Wheat Genome arrays. Expression levels of the selected genes were then evaluated by quantitative real-time PCR with cDNA samples from different tissues, stages of development and environmental conditions. Finally, fifteen novel internal control genes were selected and their respective expression profiles were compared using NormFinder, geNorm, Pearson correlation coefficients and the twofold-change method. The novel internal control genes from this study were compared with thirteen traditional ones for their expression stability. It was observed that seven of the novel internal control genes were better than the traditional ones in expression stability under all the tested cDNA samples. Among the traditional internal control genes...Continue Reading

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Citations

Sep 5, 2013·Planta·Jian MaYou-Liang Zheng
Jan 12, 2012·BMC Research Notes·Cécile PetitChristophe Délye
May 9, 2014·Applied Biochemistry and Biotechnology·Xiu-Yun YuanBo Cui
Nov 17, 2011·Biochemical and Biophysical Research Communications·Hongjian WanYuejian Yang
Jun 17, 2015·Bioscience, Biotechnology, and Biochemistry·Zubi LiuHai Liao
Jul 6, 2014·Molecular Biology Reports·Ruth PérezAlfredo De Bustos
Apr 26, 2019·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Zhiqiang WangYaxi Liu
Jun 23, 2018·Functional & Integrative Genomics·Mehdi MansouriYuichi Tada
Mar 3, 2019·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Jian YangTingjie Cao

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