Genome-wide identification and immune response analysis of serine protease inhibitor genes in the silkworm, Bombyx mori.

PloS One
Ping ZhaoQingyou Xia

Abstract

In most insect species, a variety of serine protease inhibitors (SPIs) have been found in multiple tissues, including integument, gonad, salivary gland, and hemolymph, and are required for preventing unwanted proteolysis. These SPIs belong to different families and have distinct inhibitory mechanisms. Herein, we predicted and characterized potential SPI genes based on the genome sequences of silkworm, Bombyx mori. As a result, a total of eighty SPI genes were identified in B. mori. These SPI genes contain 10 kinds of SPI domains, including serpin, Kunitz_BPTI, Kazal, TIL, amfpi, Bowman-Birk, Antistasin, WAP, Pacifastin, and alpha-macroglobulin. Sixty-three SPIs contain single SPI domain while the others have at least two inhibitor units. Some SPIs also contain non-inhibitor domains for protein-protein interactions, including EGF, ADAM_spacer, spondin_N, reeler, TSP_1 and other modules. Microarray analysis showed that fourteen SPI genes from lineage-specific TIL family and Group F of serpin family had enriched expression in the silk gland. The roles of SPIs in resisting pathogens were investigated in silkworms when they were infected by four pathogens. Microarray and qRT-PCR experiments revealed obvious up-regulation of 8, 4, 3 ...Continue Reading

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Citations

Oct 29, 2013·Annual Review of Entomology·Qingyou XiaQili Feng
Nov 29, 2014·BMC Genomics·Carolyn E RiddellEamonn B Mallon
Oct 26, 2013·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Hu WanByung Rae Jin
Jul 31, 2012·Insect Biochemistry and Molecular Biology·Youshan LiQingyou Xia
Nov 22, 2015·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Bing ZhangCongjing Feng
Aug 15, 2015·Developmental and Comparative Immunology·Pakkakul SangsuriyaPiti Amparyup
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Feb 17, 2015·Insect Science·Xiao-Ting HuaQing-You Xia
May 12, 2015·Insect Biochemistry and Molecular Biology·Desiree HarpelJames R Walters
Jul 1, 2015·Immunogenetics·Iván D OcampoLuis F Cadavid
Feb 28, 2015·Developmental and Comparative Immunology·Dongran LiuChaoliang Liu
Dec 24, 2014·Insect Biochemistry and Molecular Biology·Quanmei ChenQingyou Xia
Jul 8, 2015·Frontiers in Plant Science·Myriam SiegwartMiguel Lopez-Ferber
Mar 13, 2018·Insect Molecular Biology·I Matetovici, J Van Den Abbeele
Nov 7, 2017·PloS One·Joaquin Alonso, Manuel Martinez
Nov 14, 2019·International Journal of Molecular Sciences·Meirong ZhangGuozheng Zhang
Dec 2, 2017·Archives of Insect Biochemistry and Physiology·Saima KausarChaoliang Liu
May 30, 2019·International Journal of Molecular Sciences·Run ShiPing Zhao
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Feb 21, 2021·International Journal of Biological Macromolecules·Xiaolu ZhangPing Zhao
Jun 23, 2020·Fish & Shellfish Immunology·Yuhong ShiChenghua Li
Jul 18, 2021·Archives of Insect Biochemistry and Physiology·Tao LiMuwang Li

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Datasets Mentioned

BETA
AAB46908
GSE17571

Methods Mentioned

BETA
PCR

Software Mentioned

GENEDOC
TreeView
ClustalX
Cluster
BLAST
SeqMan
GeneCluster

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