Genome-wide identification of binding sites for the nitric oxide-sensitive transcriptional regulator NsrR

Methods in Enzymology
Sam EfromovichStephen Spiro

Abstract

NsrR is a nitric oxide-sensitive regulator of transcription. In Escherichia coli, NsrR is a repressor of the hmp gene encoding the flavohemoglobin that detoxifies nitric oxide. Three other transcription units (ytfE, ygbA, and hcp-hcr) are known to be subject to regulation by NsrR. This chapter describes experimental and statistical protocols used to identify NsrR-binding sites in the E. coli chromosome using chromatin immunoprecipitation and microarray analysis. The methods are applicable, with suitable modifications, to any regulatory protein and any organism.

Citations

Oct 30, 2012·Journal of Bacteriology·Ivan C NdamukongC Jeffrey Smith
Jul 7, 2010·BMC Genomics·Torsten Waldminghaus, Kirsten Skarstad
Sep 25, 2016·Microbiology·David C Grainger
Aug 17, 2011·Biotechnology Journal·Byung-Kwan ChoKarsten Zengler
Feb 22, 2012·Free Radical Biology & Medicine·Michael T Forrester, Matthew W Foster

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