Genome-Wide Identification of Destruxin A-Responsive Immunity-Related MicroRNAs in Diamondback Moth, Plutella xylostella

Frontiers in Immunology
Muhammad ShakeelFengliang Jin

Abstract

Plutella xylostella, a global key pest, is one of the major lepidopteran pests of cruciferous vegetables owing to its strong ability of resistance development to a wide range of insecticides. Destruxin A, a mycotoxin of the entomopathogenic fungus, Metarhizium anisopliae, has broad-spectrum insecticidal effects and has been used as an alternative control strategy to reduce harmful effects of insecticides. However, microRNA (miRNA)-regulated reactions against destruxin A have not been elucidated yet. Therefore, here, to identify immunity-related miRNAs, we constructed four small RNA libraries from destruxin A-injected larvae of P. xylostella at three different time courses (2, 4, and 6 h) with a control, and sequenced by Illumina. Our results showed that totally 187 known and 44 novel miRNAs were identified in four libraries by bioinformatic analysis. Interestingly, among differentially expressed known miRNAs, some conserved miRNAs, such as miR-263, miR-279, miR-306, miR-2a, and miR-308, predicted to be involved in regulating immunity-related genes, were also identified. Worthy to mention, miR-306 and miR-279 were also listed as common abundantly expressed miRNA in all treatments. The Kyoto Encyclopedia of Genes and Genomes path...Continue Reading

References

Jan 1, 1997·Veterinary Pathology·C S ElangbamR R Dahlgren
Feb 7, 1998·Proceedings of the National Academy of Sciences of the United States of America·B LemaitreJ A Hoffmann
Oct 27, 1999·Journal of Invertebrate Pathology·M J KershawA K Charnley
Mar 4, 2000·Insect Biochemistry and Molecular Biology·H Jiang, M R Kanost
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Feb 28, 2002·Phytochemistry·M Soledade C PedrasDale E Ward
Dec 24, 2002·Current Opinion in Immunology·Dan Hultmark
Dec 24, 2003·PLoS Biology·Alexander StarkStephen M Cohen
Jan 8, 2004·Genome Biology·Anton J EnrightDebora S Marks
Jun 18, 2004·Immunological Reviews·Håkan Steiner
Jun 18, 2004·Immunological Reviews·Michael R KanostXiao-Qiang Yu
Jun 18, 2004·Immunological Reviews·Lage Cerenius, Kenneth Söderhäll
Apr 6, 2005·Developmental Cell·Rebecca SchwabDetlef Weigel
Apr 27, 2005·Cell·Edwards AllenJames C Carrington
May 17, 2005·Insect Biochemistry and Molecular Biology·K Michel, F C Kafatos
Jun 2, 2006·RNA·Gregor ObernostererJavier Martinez
Jul 18, 2006·Nucleic Acids Research·Jan Krüger, Marc Rehmsmeier
Nov 9, 2007·Proceedings of the National Academy of Sciences of the United States of America·J Mauro CalabresePhillip A Sharp
Nov 13, 2008·Toxicon : Official Journal of the International Society on Toxinology·Qiong-Bo HuShun-Xiang Ren
Jan 12, 2010·Toxicon : Official Journal of the International Society on Toxinology·Esau Ruiz-SanchezAngela B Lange
Jan 5, 2011·Gastroenterology Research and Practice·Masahiro Yamamoto, Kiyoshi Takeda
Jul 2, 2011·Toxicon : Official Journal of the International Society on Toxinology·Xiang MengShunxiang Ren
Sep 14, 2011·Nucleic Acids Research·Marc R FriedländerNikolaus Rajewsky
Nov 15, 2011·Biotechnology Advances·Bing-Lan Liu, Yew-Min Tzeng
Mar 6, 2012·Nature Methods·Ben Langmead, Steven L Salzberg
Nov 21, 2012·Insect Biochemistry and Molecular Biology·Keira Lucas, Alexander S Raikhel
Jan 29, 2013·Insect Biochemistry and Molecular Biology·Kayvan EtebariSassan Asgari
Feb 15, 2013·The Journal of General Virology·Mohammad MehrabadiSassan Asgari
Aug 21, 2013·Virulence·Jennifer F HarrisElizabeth Hong-Geller
Aug 26, 2014·BMC Genomics·Krishnendu Mukherjee, Andreas Vilcinskas
Aug 26, 2014·Journal of Insect Physiology·Mazhar Hussain, Sassan Asgari
Feb 13, 2015·Frontiers in Microbiology·Bethany A StokesIoannis Eleftherianos
Aug 13, 2015·ELife·Vikram AgarwalDavid P Bartel

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Methods Mentioned

BETA
PCR
Illumina sequencing
RNA-Seq

Software Mentioned

Bowtie
miRDeep2
SPSS
RNAhybrid
miRanda
TargetScan
miRDeep

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