Genome-wide identification of lncRNAs associated with chlorantraniliprole resistance in diamondback moth Plutella xylostella (L.)

BMC Genomics
Bin ZhuPei Liang

Abstract

Long noncoding RNAs (lncRNAs) are now considered important regulatory factors, with a variety of biological functions in many species including insects. Some lncRNAs have the ability to show rapid responses to diverse stimuli or stress factors and are involved in responses to insecticide. However, there are no reports to date on the characterization of lncRNAs associated with chlorantraniliprole resistance in Plutella xylostella. Nine RNA libraries constructed from one susceptible (CHS) and two chlorantraniliprole-resistant P. xylostella strains (CHR, ZZ) were sequenced, and 1309 lncRNAs were identified, including 877 intergenic lncRNAs, 190 intronic lncRNAs, 76 anti-sense lncRNAs and 166 sense-overlapping lncRNAs. Of the identified lncRNAs, 1059 were novel. Furthermore, we found that 64 lncRNAs were differentially expressed between CHR and CHS and 83 were differentially expressed between ZZ and CHS, of which 22 were differentially expressed in both CHR and ZZ. Most of the differentially expressed lncRNAs were hypothesized to be associated with chlorantraniliprole resistance in P. xylostella. The targets of lncRNAs via cis- (<10 kb upstream and downstream) or trans- (Pearson's correlation, r > 0.9 or < -0.9, P < 0.05) regulator...Continue Reading

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Citations

Mar 31, 2018·Mini Reviews in Medicinal Chemistry·Zhiqiang Sun, Hui Xu
Nov 2, 2019·Archives of Insect Biochemistry and Physiology·Ruobing GuanShiheng An
May 3, 2019·Wiley Interdisciplinary Reviews. RNA·Luis María Vaschetto, Hernán Mario Beccacece
Nov 15, 2019·Cellular and Molecular Life Sciences : CMLS·Jiasheng Song, Shutang Zhou
Mar 19, 2020·BioMed Research International·Lian WangXian Jiang
May 1, 2021·Animals : an Open Access Journal From MDPI·Chhavi ChoudharyJayendra Nath Shukla
Jul 25, 2021·Pesticide Biochemistry and Physiology·Bai-Zhong ZhangXi-Ling Chen
Mar 10, 2018·Journal of Agricultural and Food Chemistry·John E Casida

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Methods Mentioned

BETA
RNA-seq
HT-Seq
PCR

Software Mentioned

DESeq
DESeq R package
Cuffcompare
PLEK
CPC
CNCI
Pfam
Agilent GeneSpring GX
Cufflinks package
NGS QC Toolkit

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